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Aptamer-mediated indirect quantum dot labeling and fluorescent imaging of target proteins in living cells

机译：适体介导的间接量子点标记和活细胞中靶蛋白的荧光成像

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摘要

Protein labeling for dynamic living cell imaging plays a significant role in basic biological research, as well as in clinical diagnostics and therapeutics. We have developed a novel strategy in which the dynamic visualization of proteins within living cells is achieved by using aptamers as mediators for indirect protein labeling of quantum dots (QDs). With this strategy, the target protein angiogenin was successfully labeled with fluorescent QDs in a minor intactness model, which was mediated by the aptamer AL6-B. Subsequent living cell imaging analyses indicated that the QDs nanoprobes were selectively bound to human umbilical vein endothelial cells, gradually internalized into the cytoplasm, and mostly localized in the lysosome organelle, indicating that the labeled protein retained high activity. Compared with traditional direct protein labeling methods, the proposed aptamer-mediated strategy is simple, inexpensive, and provides a highly selective, stable, and intact labeling platform that has shown great promise for future biomedical labeling and intracellular protein dynamic analyses.
机译：用于动态活细胞成像的蛋白质标记在基础生物学研究以及临床诊断和治疗中起着重要作用。我们已经开发了一种新颖的策略，其中通过使用适体作为量子点（QD）间接蛋白质标记的介体，实现了活细胞内蛋白质的动态可视化。通过这种策略，目标蛋白血管生成素已成功地在未修饰的完整模型中用荧光QD标记，该模型由适体AL6-B介导。随后的活细胞成像分析表明，QDs纳米探针与人脐静脉内皮细胞选择性结合，逐渐内化到细胞质中，并且大部分定位在溶酶体细胞器中，表明标记的蛋白质保留了高活性。与传统的直接蛋白质标记方法相比，拟议的适体介导的策略简单，便宜，并提供了高度选择性，稳定和完整的标记平台，为未来的生物医学标记和细胞内蛋白质动态分析显示了广阔的前景。

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