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首页> 外文期刊>Nanotechnology >Highly sensitive colorimetric detection of glucose in a serum based on DNA-embeded Au@Ag core-shell nanoparticles
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Highly sensitive colorimetric detection of glucose in a serum based on DNA-embeded Au@Ag core-shell nanoparticles

机译：基于DNA嵌入的Au @ Ag核壳纳米粒子的血清中葡萄糖的高灵敏比色检测

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摘要

Glucose is a key energy substance in diverse biology and closely related to the life activities of the organism. To develop a simple and sensitive method for glucose detection is extremely urgent but still remains a key challenge. Herein, we report a colorimetric glucose sensor in a homogeneous system based on DNA-embedded core-shell Au@Ag nanoparticles. In this assay, a glucose substrate was first catalytically oxidized by glucose oxidase to produce H2O2 which would further oxidize and gradually etch the outer silver shell of Au@Ag nanoparticles. Afterwards, the solution color changed from yellow to red and the surface plasmon resonance (SPR) band of Au@Ag nanoparticles declined and red-shifted from 430 to 516 nm. Compared with previous silver-based glucose colorimetric detection strategies, the distinctive SPR band change is superior to the color variation, which is critical to the high sensitivity of this assay. Benefiting from the outstanding optical property, robust stability and well-dispersion of the core-shell Au@AgNPs hybrid, this colorimetric assay obtained a detection limit of glucose as low as 10 nM, which is at least a 10-fold improvement over other AgNPs-based procedures. Moreover, this optical biosensor was successfully employed to the determination of glucose in fetal bovine serum.
机译：葡萄糖是多种生物学中的关键能量物质，与生物的生命活动密切相关。开发一种简单而灵敏的葡萄糖检测方法非常紧迫，但仍然是关键的挑战。在这里，我们报告在基于DNA嵌入的核壳Au @ Ag纳米粒子的同质系统中的比色葡萄糖传感器。在该测定中，首先通过葡萄糖氧化酶将葡萄糖底物催化氧化以产生H2O2，H2O2将进一步氧化并逐渐蚀刻Au @ Ag纳米颗粒的外银壳。之后，溶液的颜色由黄色变为红色，Au @ Ag纳米粒子的表面等离振子共振（SPR）带下降，并从430 nm移至516 nm。与以前的银基葡萄糖比色检测策略相比，独特的SPR谱带变化优于颜色变化，这对于此测定法的高灵敏度至关重要。得益于核壳型Au @ AgNPs杂种的出色光学性能，稳健的稳定性和良好的分散性，这种比色测定获得了低至10 nM的葡萄糖检测限，比其他AgNPs至少提高了10倍基于程序。此外，该光学生物传感器已成功地用于测定胎牛血清中的葡萄糖。

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