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首页> 外文期刊>Nanotechnology >Effect of HSA coated iron oxide labeling on human umbilical cord derived mesenchymal stem cells
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Effect of HSA coated iron oxide labeling on human umbilical cord derived mesenchymal stem cells

机译：HSA涂层氧化铁标记对人脐带间充质干细胞的影响

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摘要

Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) are known for self-renewal and differentiation into cells of various lineages like bone, cartilage and fat. They have been used in biomedical applications to treat degenerative disorders. However, to exploit the therapeutic potential of stem cells, there is a requirement of sensitive non-invasive imaging techniques which will offer the ability to track transplanted cells, bio-distribution, proliferation and differentiation. In this study, we have analyzed the efficacy of human serum albumin coated iron oxide nanoparticles (HSA-IONPs) on the differentiation of hUC-MSCs. The colloidal stability of the HSA-IONPs was tested over a long period of time (>= 20 months) and the optimized concentration of HSA-IONPs for labeling the stem cells was 60 mu g ml(-1). Detailed in vitro assays have been performed to ascertain the effect of the nanoparticles (NPs) on stem cells. Lactate dehydrogenase (LDH) assay showed minimum release of LDH depicting the least disruptions in cellular membrane. At the same time, mitochondrial impairment of the cells was also not observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry analysis revealed lesser generation of reactive oxygen species in HSA-IONPs labeled hUC-MSCs in comparison to bare and commercial IONPs. Transmission electron microscopy showed endocytic engulfment of the NPs by the hUC-MSCs. During the process, the gross morphologies of the actin cytoskeleton were found to be intact as shown by immunofluorescence microscopy. Also, the engulfment of the HSA-IONPs did not show any detrimental effect on the differentiation potential of the stem cells into adipocytes, osteocytes and chondrocytes, thereby confirming that the inherent properties of stem cells were maintained.
机译：人脐带间充质干细胞（hUC-MSC）能够自我更新并分化为各种谱系（如骨骼，软骨和脂肪）的细胞。它们已在生物医学应用中用于治疗退行性疾病。然而，为了开发干细胞的治疗潜力，需要灵敏的非侵入性成像技术，该技术将提供跟踪移植细胞，生物分布，增殖和分化的能力。在这项研究中，我们分析了人类血清白蛋白涂层的氧化铁纳米颗粒（HSA-IONPs）对hUC-MSC分化的功效。在长时间（> = 20个月）内测试了HSA-IONPs的胶体稳定性，用于标记干细胞的HSA-IONPs的最佳浓度为60μg ml（-1）。已经进行了详细的体外测定，以确定纳米颗粒（NP）对干细胞的作用。乳酸脱氢酶（LDH）分析显示LDH释放最少，表明细胞膜破坏最少。同时，也未通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）测定法观察到细胞的线粒体损伤。流式细胞仪分析表明，与裸和商业IONP相比，HSA-IONPs标记的hUC-MSC中活性氧的生成较少。透射电子显微镜显示hUC-MSCs吞噬了NP。在该过程中，如免疫荧光显微镜检查所示，发现肌动蛋白细胞骨架的总体形态是完整的。同样，HSA-IONPs的吞噬对干细胞向脂肪细胞，骨细胞和软骨细胞的分化潜能没有显示出任何有害作用，从而证实了干细胞的固有特性得以维持。

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