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Surveying colloid sedimentation by coplanar waveguides

机译:通过共面波导测量胶体沉积

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By using coplanar waveguides, direct access to the dielectric properties of aqueous solutions of polystyrene beads with different diameters from 330 nm to 10 mu m is provided. The relative variation of the transmission parameter with respect to water is monitored, ranging from similar to 3% obtained for a 9.5% solution with 330 nm diameter beads to similar to 22% for 10 mu m diameter particles at the same concentration. To highlight its applicability in biosensing, the technique was further employed to survey the clustering between biotin and streptavidin-coated beads. The transmission parameter displays a similar to 50% increase for mixtures containing nine volumes of biotin and one volume of streptavidin-modified beads (4.5 ng mu l(-1) of streptavidin) and reaches similar to 400% higher values when equal volumes of biotin and streptavidin-coated beads (22.5 ng mu l(-1) of streptavidin) were mixed.
机译:通过使用共面波导,可以直接访问直径从330 nm到10μm的聚苯乙烯珠粒水溶液的介电性能。监测透射参数相对于水的相对变化,其范围从在具有相同浓度的直径为330 nm的9.5%溶液中获得的3%到在直径为10μm的颗粒中达到22%的相似(约22%)。为了突出其在生物传感中的适用性,该技术被进一步用于调查生物素和链霉亲和素包被的磁珠之间的聚集。对于包含九体积生物素和一体积抗生蛋白链菌素修饰的珠子(4.5纳克微升(-1)抗生蛋白链菌素)的混合物,透射参数显示出类似于增加50%的值,而当相等体积的生物素时,传递参数显示出接近400%的高值混合链霉亲和素包被的珠子(链霉亲和素22.5 ngμl(-1))。

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