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Polyethylenimine mediated silver nanoparticle-decorated magnetic graphene as a promising photothermal antibacterial agent

机译:聚乙烯亚胺介导的银纳米粒子修饰磁性石墨烯作为一种有前途的光热抗菌剂

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摘要

A novel bactericidal material, Ag@rGO-Fe3O4-PEI composite is prepared by in situ growth of silver nanoparticles onto the polyethylenimine (PEI)-mediated magnetic reduced graphene oxide (GO). The antibacterial performances of the composite are investigated by using the gram-negative bacteria Escherichia coli O157:H7 (E. coli O157: H7) as a model. The results indicate that the Ag@rGO-Fe3O4-PEI composite exhibits excellent antibacterial performance against E. coli O157:H7, with an antibacterial performance superior to those for the ever-reported photothermal materials. The bactericidal capability or the inhibition capability for bacteria growth is found to depend on the dosage of the Ag@rGO-Fe3O4-PEI and Ag/rGO-Fe3O4-PEI mass ratio within a certain range. By using a dosage of 0.1 mu gmL(-1), a killing rate of 99.9% is achieved for the E. coli O157: H7 (1 x 107 cfu mL(-1)) under a 0.5 min NIR laser irradiation (785 nm/50 mW cm(-2)). In addition, a minimum bactericidal concentration (MBC) of 0.100 mu gmL(-1) is achieved under near infrared (NIR) laser irradiation for 10 min, for which case there is absolutely no colony of E. coli O157:H7 found in the broth agar plate.
机译:通过将银纳米颗粒原位生长到聚乙烯亚胺(PEI)介导的磁性还原氧化石墨烯(GO)上,制备了新型杀菌材料Ag @ rGO-Fe3O4-PEI复合材料。以革兰氏阴性细菌大肠杆菌O157:H7(大肠杆菌O157:H7)为模型,研究了复合材料的抗菌性能。结果表明,Ag @ rGO-Fe3O4-PEI复合材料对大肠杆菌O157:H7表现出优异的抗菌性能,其抗菌性能优于以往报道的光热材料。发现对细菌生长的杀菌能力或抑制能力取决于Ag @ rGO-Fe3O4-PEI和Ag / rGO-Fe3O4-PEI质量比的剂量在一定范围内。通过使用0.1μgmL(-1)的剂量,在0.5分钟的近红外激光照射(785 nm)下,对O157:H7大肠杆菌(1 x 107 cfu mL(-1))的杀灭率达到99.9% / 50 mW cm(-2))。此外,在近红外(NIR)激光辐照10分钟的情况下,最小杀菌浓度(MBC)为0.100μgmL(-1),在这种情况下,绝对不会在大肠杆菌中发现O157:H7菌落。肉汤琼脂板。

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