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Magnetic apatite for structural insights on the plasma membrane

机译:磁性磷灰石对质膜的结构影响

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The iron oxide-hydroxyapatite (FeOxHA) nanoparticles reported here differ from those reported before by their advantage of homogeneity and simple preparation; moreover, the presence of carboxymethyldextran (CMD), together with hydroxyapatite (HA), allows access to the cellular membrane, which makes our magnetic apatite unique. These nanoparticles combine magnetic behavior, Raman label ability and the property of interaction with the cellular membrane; they therefore represent an interesting material for structural differentiation of the cell membrane. It was observed by Raman spectroscopy, scanning electron microscopy (SEM) and fluorescence microscopy that FeOxHA adheres to the plasma membrane and does not penetrate the membrane. These insights make the nanoparticles a promising material for magnetic cell sorting, e.g. in microfluidic device applications.
机译:此处报道的氧化铁-羟基磷灰石(FeOxHA)纳米颗粒与以前报道的纳米颗粒具有均一性和易于制备的优点。此外,羧甲基葡聚糖(CMD)和羟基磷灰石(HA)的存在可以进入细胞膜,这使我们的磁性磷灰石独树一帜。这些纳米粒子结合了磁行为,拉曼标记能力和与细胞膜相互作用的特性。因此,它们代表了用于细胞膜结构分化的有趣材料。通过拉曼光谱,扫描电子显微镜(SEM)和荧光显微镜观察到,FeOxHA附着在质膜上并且不穿透该膜。这些见解使纳米颗粒成为用于磁性细胞分选的有前途的材料,例如。在微流控设备中的应用。

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