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首页> 外文期刊>Neuroscience Letters: An International Multidisciplinary Journal Devoted to the Rapid Publication of Basic Research in the Brain Sciences >Mechanism of soluble beta-amyloid 25-35 neurotoxicity in primary cultured rat cortical neurons
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Mechanism of soluble beta-amyloid 25-35 neurotoxicity in primary cultured rat cortical neurons

机译：可溶性β-淀粉样蛋白25-35在原代培养的大鼠皮质神经元中的神经毒性机制

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摘要

This study aimed to determine the effects of different concentrations of soluble beta-amyloid 25-35 (A beta 25-35) on cell viability, calcium overload, and PI3K-p85 expression in cultured cortical rat neurons. Primary cultured cerebral cortical neurons of newborn rats were divided randomly into six groups. Five groups were treated with soluble A beta 25-35 at concentrations of 10 nmol/L, 100 nmol/L, 1 mu mol/L, 10 mu mol/L, or 30 mu mol/L. Cell Counting Kit-8 staining was used to measure cell viability, laser-scanning confocal imaging was used to detect changes in intracellular free calcium concentration, and western blot assay was used to measure neuronal PI3K-p85 expression. Soluble A beta 25-35 was found to reduce cell viability and induce calcium overload in primary cultured rat cerebral cortical neurons, in a concentration-dependent manner. At certain concentrations, soluble A beta 25-35 also increased neuronal PI3K-p85 expression. These findings reveal that soluble A beta 25-35 reduces the viability of cultured cerebral cortical rat neurons. The neurotoxicity mechanism may involve calcium overload and disruption of insulin signal transduction pathways. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
机译：这项研究旨在确定不同浓度的可溶性β-淀粉样蛋白25-35（A beta 25-35）对培养的皮质大鼠神经元细胞活力，钙超载和PI3K-p85表达的影响。将新生大鼠的原代培养的大脑皮层神经元随机分为六组。五组分别用浓度为10 nmol / L，100 nmol / L，1μmol/ L，10μmol/ L或30μmol/ L的可溶性A beta 25-35处理。 Cell Counting Kit-8染色用于测量细胞活力，激光扫描共聚焦成像用于检测细胞内游离钙浓度的变化，Western印迹测定用于测量神经元PI3K-p85的表达。发现可溶性A beta 25-35以浓度依赖的方式降低原代培养的大鼠大脑皮质神经元的细胞活力并诱导钙超载。在某些浓度下，可溶性A beta 25-35也会增加神经元PI3K-p85的表达。这些发现表明，可溶性A beta 25-35降低了培养的大脑皮层大鼠神经元的活力。神经毒性机制可能涉及钙超载和胰岛素信号转导途径的破坏。 （C）2016 Elsevier Ireland Ltd.保留所有权利。

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