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首页> 外文期刊>Neuroscience: An International Journal under the Editorial Direction of IBRO >IMAGING MASS SPECTROMETRY DETECTS DYNAMIC CHANGES OF PHOSPHATIDYLCHOLINE IN RAT HIPPOCAMPAL CA1 AFTER TRANSIENT GLOBAL ISCHEMIA
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IMAGING MASS SPECTROMETRY DETECTS DYNAMIC CHANGES OF PHOSPHATIDYLCHOLINE IN RAT HIPPOCAMPAL CA1 AFTER TRANSIENT GLOBAL ISCHEMIA

机译：影像质谱法检测短暂性全脑缺血后大鼠海马CA1中磷脂酰胆碱的动态变化

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Background and purpose: The initial steps in the cascade leading to cell death are still unknown because of the limitations of the existing methodology, strategy, and modalities used. Methods: Imaging mass spectrometry (IMS) was used to measure dynamic molecular changes of phosphatidylcholine (PC) species in the rat hippocampus after transient global ischemia (TGI) for 6 min. Fresh frozen sections were obtained after euthanizing the rats on Days 1, 2, 4, 7, 10, 14, and 21. Histopathology and IMS of adjacent sections compared morphological and molecular changes, respectively. Results: Histopathological changes were absent immediately after TGI (at Day 1, superacute phase). At Days 2-21 after TGI (from subacute to chronic phases), histopathology revealed neuronal death associated with gliosis, inflammation, and accumulation of activated microglia in CA1. IMS detected significant molecular changes after TGI in the same CA1 domain: increase of PC (diacyl-16: 0/22: 6) in the superacute phase and increase of PC (diacyl-16:0/18:1) in the subacute to chronic phases. Conclusions: Histopathology and IMS can provide comprehensive and complementary information on cell death mechanisms in the hippocampal CA1 after global ischemia. IMS provided novel data on molecular changes in phospholipids immediately after TGI. Increased level of PC (diacyl-16: 0/22: 6) in the pyramidal cell layer of hippocampal CA1 prior to the histopathological change may represent an early step in delayed neuronal death mechanisms. (C) 2016 The Authors. Published by Elsevier Ltd. on behalf of IBRO.
机译：背景和目的：由于所使用的现有方法，策略和方式的局限性，导致细胞死亡的级联反应的初始步骤仍然未知。方法：采用成像质谱（IMS）法测定短暂性全脑缺血（TGI）6分钟后大鼠海马中磷脂酰胆碱（PC）种类的动态分子变化。在第1、2、4、7、10、14和21天对大鼠实施安乐死后，可获得新鲜的冷冻切片。相邻切片的组织病理学和IMS分别比较了形态和分子变化。结果：TGI后（第1天，超急性期）立即没有组织病理学改变。在TGI后的2-21天（从亚急性到慢性期），组织病理学显示与CA1胶质增生，炎症和活化的小胶质细胞积聚有关的神经元死亡。 IMS检测到TGI后在相同的CA1域中发生了显着的分子变化：超急性期PC（diacyl-16：0/22：6）升高，亚急性期PC（diacyl-16：0/18：1）升高。慢性期。结论：组织病理学和IMS可以提供​​关于全脑缺血后海马CA1细胞死亡机制的全面补充信息。 IMS在TGI之后立即提供了有关磷脂分子变化的新数据。在组织病理学改变之前，海马CA1锥体细胞层中PC水平的升高（diacyl-16：0/22：6）可能代表了神经元死亡机制延迟的早期步骤。 （C）2016作者。由Elsevier Ltd.代表IBRO发布。

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