Histone H1 null vertebrate cells exhibit altered nucleosome architecture

Hashimoto Hideharu; Takami Yasunari; Sonoda Eiichiro; Iwasaki Tomohito; Iwano Hidetomo; Tachibana Makoto; Takeda Shunichi; Nakayama Tatsuo; Kimura Hiroshi; Shinkai Yoichi

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Histone H1 null vertebrate cells exhibit altered nucleosome architecture

机译：组蛋白H1空脊椎动物细胞显示出改变的核小体结构

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In eukaryotic nuclei, DNA is wrapped around an octamer of core histones to form nucleosomes, and chromatin fibers are thought to be stabilized by linker histones of the H1 type. Higher eukaryotes express multiple variants of histone H1; chickens possess six H1 variants. Here, we generated and analyzed the phenotype of a complete deletion of histone H1 genes in chicken cells. The H1-null cells showed decreased global nucleosome spacing, expanded nuclear volumes, and increased chromosome aberration rates, although proper mitotic chromatin structure appeared to be maintained. Expression array analysis revealed that the transcription of multiple genes was affected and was mostly downregulated in histone H1-deficient cells. This report describes the first histone H1 complete knockout cells in vertebrates and suggests that linker histone H1, while not required for mitotic chromatin condensation, plays important roles in nucleosome spacing and interphase chromatin compaction and acts as a global transcription regulator.

机译：在真核中，DNA被包裹在核心组蛋白的八聚体周围以形成核小体，染色质纤维被认为是由H1型接头组蛋白稳定的。高等真核生物表达组蛋白H1的多种变体。鸡具有六个H1变体。在这里，我们生成并分析了鸡细胞中组蛋白H1基因完全缺失的表型。 H1无细胞显示减少整体核小体间距，扩大核体积和增加染色体畸变率，虽然似乎保持适当的有丝分裂染色质结构。表达阵列分析显示，多个基因的转录受到影响，并且在组蛋白H1缺陷型细胞中大部分被下调。该报告描述了脊椎动物中第一个组蛋白H1完整的敲除细胞，并表明接头组蛋白H1虽然不是有丝分裂染色质凝聚所必需的，但在核小体间距和相间染色质紧实中起着重要作用，并起着整体转录调节剂的作用。

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《Nucleic Acids Research》 |2010年第11期|共13页
作者
Hashimoto Hideharu; Takami Yasunari; Sonoda Eiichiro; Iwasaki Tomohito; Iwano Hidetomo; Tachibana Makoto; Takeda Shunichi; Nakayama Tatsuo; Kimura Hiroshi; Shinkai Yoichi;
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正文语种 eng
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phenotype; chromosome aberration; chromatin structure; nuclear volume; nucleosome spacing;

机译：表型染色体畸变染色质结构核体积核小体间距;

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1. Histone H1 null vertebrate cells exhibit altered nucleosome architecture [J] . Hashimoto Hideharu, Takami Yasunari, Sonoda Eiichiro, Nucleic Acids Research . 2010,第11期

机译：组蛋白H1空脊椎动物细胞显示出改变的核小体结构
2. Altered Expression of Nuclear and Cytoplasmic Histone H1 in Pulmonary Artery and Pulmonary Artery Smooth Muscle Cells in Patients with IPAH: [J] . Megha Talati, Erin Seeley, Kaori Ihida-Stansbury, Pulmonary Circulation . 2012,第3期

机译：IPAH患者肺动脉和肺动脉平滑肌细胞中核和细胞质组蛋白H1表达的改变：
3. Altered Expression of Nuclear and Cytoplasmic Histone H1 in Pulmonary Artery and Pulmonary Artery Smooth Muscle Cells in Patients with IPAH: [J] . Megha Talati, Erin Seeley, Kaori Ihida-Stansbury, Pulmonary Circulation . 2012,第3期

机译：IPAH患者肺动脉和肺动脉平滑肌细胞中核和细胞质组蛋白H1表达的改变：
4. Phosphoproteomic Analysis of Linker Histone H1 from Growing and Starved Tetrahymena Cells [C] . Benjamin A. Garcia, Swati Joshi, C. Eric Thomas, ASMS Conference on Mass Spectrometry and Allied Topics . 2006

机译：增长和饥饿的四氢钠细胞的接头组蛋白H1的磷蛋白酶分析
5. Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-Induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells. [D] . Schauwecker, Suzanne Marie. 2017

机译：组蛋白H1和染色体蛋白HMGN2调节催乳素诱导的STAT5转录因子在乳腺癌细胞中的募集和功能。
6. Histone H1 null vertebrate cells exhibit altered nucleosome architecture [O] . Hideharu Hashimoto, Yasunari Takami, Eiichiro Sonoda, 2010

机译：组蛋白H1无脊椎动物细胞显示出改变的核小体结构
7. Histone H1 null vertebrate cells exhibit altered nucleosome architecture [O] . Hashimoto, Hideharu, Takami, Yasunari, Sonoda, Eiichiro, 2010

机译：组蛋白H1无脊椎动物细胞显示出改变的核小体结构

Histone H1 null vertebrate cells exhibit altered nucleosome architecture

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