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首页> 外文期刊>Nucleic Acids Research >[C(7)GC(4)](4) Association into supra molecular i-motif structures
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[C(7)GC(4)](4) Association into supra molecular i-motif structures

机译：[C（7）GC（4）]（4）与超分子i-基序结构缔合

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摘要

The self-associative properties of cytidine-rich oligonucleotides into symmetrical i-motif tetramers give to these oligonucleotides the capacity of forming supramolecular structures (sms) that have potential applications in the nanotechnology domain. In order to facilitate sms formation, oligonucleotides containing two cytidine stretches of unequal length (CnXCm) separated by a non-cytidine spacer were synthesized. They were designed to associate into a tetramer including an i-motif core built by intercalation of the C center dot C+ pairs of the longer C stretch with the two dangling non-intercalated strands of the shorter C stretch at each end. Gel filtration chromatography shows that the non-intercalated C-rich ends give to this structure the capacity of forming extremely stable sms. Using C(7)GC(4) as a model, we find that the sms formation rate varies as the oligonucleotide concentration and increases at high temperature. Competitively with the tetramer involved in sms elongation, CnXCm oligonucleotides form i-motif dimers that compete with sms elongation. The dimer stability is strongly reduced when the pH is moved away from the cytidine pK. This results in an equilibrium shift towards the tetramer and in the acceleration of the sms formation rate. The chromatograms of the sms formed by C(7)GC(4) indicate a broad distribution. In a 1.5 mM solution incubated at 37 degrees C, the equilibrium distribution is centered on a molecular weight corresponding to the assembly of nine tetramers and the upper limit corresponds to 80 tetramers. The lifetime of this structure is about 4 days at 40 degrees C, pH 4.6.
机译：富含胞苷的寡核苷酸到对称的i-基序四聚体中的自缔合特性使这些寡核苷酸具有形成超分子结构（sms）的能力，这些超分子结构在纳米技术领域具有潜在的应用前景。为了促进sms的形成，合成了包含两个被非胞嘧啶间隔子隔开的不等长的胞嘧啶片段（CnXCm）的寡核苷酸。它们被设计成与四聚体缔合，该四聚体包括通过插入较长C链的C中心点C +对与两端较短C链的两个悬挂的非插入链而构建的i-基序核心。凝胶过滤色谱法显示，未插入的富C末端使该结构具有形成极其稳定的sms的能力。使用C（7）GC（4）作为模型，我们发现sms形成速率随寡核苷酸浓度的变化而变化，并在高温下增加。与参与sms延伸的四聚体竞争，CnXCm寡核苷酸形成与sms延伸竞争的i-motif二聚体。当pH从胞苷pK移开时，二聚体的稳定性会大大降低。这导致向四聚体的平衡移动以及sms形成速率的加速。由C（7）GC（4）形成的sm的色谱图表明分布较宽。在37摄氏度下温育的1.5 mM溶液中，平衡分布的中心是对应于九个四聚体组装体的分子量，上限对应于80个四聚体。该结构的寿命在40摄氏度，pH 4.6下约为4天。

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