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首页> 外文期刊>Nucleic Acids Research >The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos
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The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos

机译：X连锁的印迹基因家族Fthl17在小鼠胚胎的两细胞阶段后主要显示雌性表达

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Differences between male and female mammals are initiated by embryonic differentiation of the gonad into either a testis or an ovary. However, this may not be the sole determinant. There are reports that embryonic sex differentiation might precede and be independent of gonadal differentiation, but there is little molecular biological evidence for this. To test for sex differences in early-stage embryos, we separated male and female blastocysts using newly developed non-invasive sexing methods for transgenic mice expressing green fluorescent protein and compared the gene-expression patterns. From this screening, we found that the Fthl17 (ferritin, heavy polypeptide-like 17) family of genes was predominantly expressed in female blastocysts. This comprises seven genes that cluster on the X chromosome. Expression analysis based on DNA polymorphisms revealed that these genes are imprinted and expressed from the paternal X chromosome as early as the two-cell stage. Thus, by the time zygotic genome activation starts there are already differences in gene expression between male and female mouse embryos. This discovery will be important for the study of early sex differentiation, as clearly these differences arise before gonadal differentiation.
机译：雄性和雌性哺乳动物之间的差异是由性腺的胚胎分化为睾丸或卵巢引起的。但是，这可能不是唯一的决定因素。有报道说，胚胎的性别分化可能先于性分化，而与性腺分化无关，但对此没有分子生物学证据。为了测试早期胚胎中的性别差异，我们使用新开发的非侵入性性别鉴定方法对表达绿色荧光蛋白的转基因小鼠分离了男性和女性胚泡，并比较了基因表达模式。通过此筛选，我们发现Fthl17（铁蛋白，重多肽样17）基因家族主要在女性胚泡中表达。它包含七个聚集在X染色体上的基因。基于DNA多态性的表达分析表明，这些基因早在两细胞阶段就已从父系X染色体上印记并表达。因此，到合子基因组激活开始时，雄性和雌性小鼠胚胎之间的基因表达已经存在差异。这一发现对于早期性别分化的研究将是重要的，因为显然这些差异是在性腺分化之前出现的。

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