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首页> 外文期刊>Nucleic Acids Research >CPEB2, CPEB3 and CPEB4 are coordinately regulated by miRNAs recognizing conserved binding sites in paralog positions of their 3 '-UTRs
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CPEB2, CPEB3 and CPEB4 are coordinately regulated by miRNAs recognizing conserved binding sites in paralog positions of their 3 '-UTRs

机译：通过识别其3'-UTR旁系同源位点的保守结合位点的miRNA协调调控CPEB2，CPEB3和CPEB4

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摘要

The cytoplasmic polyadenylation element binding-protein (CPEB) is an RNA-binding protein that participates in translational control. CPEB2, CPEB3 and CPEB4 are paralog proteins very similar among themselves referred as the CPEB2 subfamily. To gain insight into common mechanisms of regulation of the CPEB2 subfamily transcripts, we looked for putative cis-acting elements present in the 3'-UTRs of the three paralogs. We found different families of miRNAs predicted to target all subfamily members. Most predicted target sites for these families are located in paralog positions suggesting that these putative regulatory motifs were already present in the ancestral gene. We validated target sites for miR-92 and miR-26 in the three paralogs using mutagenesis of miRNA-binding sites in reporter constructs combined with over-expression and depletion of miRNAs. Both miR-92 and miR-26 induced a decrease in Luciferase activity associated to a reduction in mRNA levels of the reporter constructs. We also showed that the endogenous miRNAs co-regulate CPEB2, CPEB3 and CPEB4 transcripts, supporting our hypothesis that these genes have a common regulatory mechanism mediated by miRNAs. We also suggest that the ancestral pattern of miRNA-binding motifs was maintained throughout the generation of highly conserved elements in each of the 3'-UTRs.
机译：细胞质聚腺苷酸化元件结合蛋白（CPEB）是一种RNA结合蛋白，参与翻译控制。 CPEB2，CPEB3和CPEB4是彼此之间非常相似的旁系同源蛋白，被称为CPEB2亚家族。为了深入了解CPEB2亚家族转录物调控的常见机制，我们寻找了三个旁系同源物3'-UTR中存在的假定的顺式作用元件。我们发现了预测针对所有亚家族成员的不同miRNA家族。这些家族的大多数预测目标位点位于旁系同源位置，表明这些推定的调控基序已经存在于祖先基因中。我们通过诱变报告基因构建体中的miRNA结合位点与miRNA的过度表达和耗竭相结合，在三个旁系同源物中验证了miR-92和miR-26的靶位点。 miR-92和miR-26均可诱导萤光素酶活性降低，这与报告基因构建体mRNA水平的降低有关。我们还表明，内源性miRNA共同调节CPEB2，CPEB3和CPEB4转录本，支持我们的假设，即这些基因具有由miRNA介导的共同调控机制。我们还建议，miRNA结合基序的祖先模式在每个3'-UTRs中高度保守的元素的生成过程中都得到了维持。

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