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首页> 外文期刊>Langmuir: The ACS Journal of Surfaces and Colloids >Viscoelastic measurements of single molecules on a millisecond time scale by magnetically driven oscillation of an atomic force microscope cantilever
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Viscoelastic measurements of single molecules on a millisecond time scale by magnetically driven oscillation of an atomic force microscope cantilever

机译：通过原子力显微镜悬臂的磁驱动振荡，在毫秒级上对单个分子进行粘弹性测量

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The dynamical nature of biomolecular systems means that knowledge of their viscoelastic behavior is important in fully understanding function. The linear viscoelastic response can be derived from an analysis of Brownian motion. However, this is a slow measurement and technically demanding for many molecular systems of interest. To address this issue, we have developed a simple method for measuring the full linear viscoelastic response of single molecules based on magnetically driven oscillations of an atomic force microscope cantilever. The cantilever oscillation frequency is periodically swept through the system resonance in less than 200 ms allowing the power spectrum to be obtained rapidly and analyzed with a suitable model. The technique has been evaluated using dextran, a polysaccharide commonly used as a test system for single molecule mechanical manipulation experiments. The monomer stiffness and friction constants were compared with those derived from other methods. Excellent agreement is obtained indicating that the new method accurately and, most importantly, rapidly provides the viscoelastic response of a single molecule between the tip and substrate. The method will be a useful tool for studying systems that change their structure and dynamic response on a time scale of 100-200 ms, such as protein folding and unfolding under applied force.
机译：生物分子系统的动力学性质意味着，对它们的粘弹性行为的了解对于充分理解功能至关重要。线性粘弹性响应可以从布朗运动的分析中得出。但是，这是一个缓慢的测量，并且对许多目标分子系统在技术上都有要求。为了解决这个问题，我们开发了一种简单的方法，用于基于原子力显微镜悬臂的磁驱动振荡来测量单个分子的全线性粘弹性响应。悬臂振荡频率在不到200 ms的时间内周期性地扫过系统谐振，从而可以快速获得功率谱并使用合适的模型进行分析。该技术已使用葡聚糖（一种通常用作单分子机械操作实验的测试系统的多糖）进行了评估。单体刚度和摩擦常数与其他方法进行了比较。获得了极好的一致性，表明新方法能够准确地（最重要的是迅速地）提供尖端和基底之间单个分子的粘弹性响应。该方法将是研究系统在100-200 ms的时间范围内改变其结构和动态响应（例如在施加力的情况下蛋白质折叠和展开）的有用工具。

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