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首页> 外文期刊>Langmuir: The ACS Journal of Surfaces and Colloids >Vesicle fission of giant unilamellar vesicles of liquid-ordered-phase membranes induced by amphiphiles with a single long hydrocarbon chain
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Vesicle fission of giant unilamellar vesicles of liquid-ordered-phase membranes induced by amphiphiles with a single long hydrocarbon chain

机译：具有单条长烃链的两亲物诱导的液相有序相膜的巨大单层囊泡的囊泡裂变

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摘要

Vesicle fissions are very important processes of biomembranes in cells, but their mechanisms are not clear and are controversial. Using the single giant unilamellar vesicle (GUV) method, we recently found that low concentrations (less than the critical micelle concentration (CMC)) of lysophosphatidylcholine (lyso-PC) induced the vesicle fission of GUVs of dipalmitoylphosphatidylcholine/cholesterol(6/4) (DPPC/chol(6/4)) membranes and sphingomyelin/cholesterol membranes (6/4) in the liquid-ordered (lo) phase. In this report, to elucidate its mechanism, we have investigated the effect of low concentrations (much less than their CMC) of other amphiphiles with a single long hydrocarbon chain (i.e., single long chain amphiphiles) on DPPC/chol(6/4) GUVs as well as the effect of the membrane composition on the lyso-PC-induced vesicle fission. We found that low concentrations of single long chain amphiphiles (lyosophosphatidic acid, octylglucoside, and sodium dodecyl sulfate) induced the shape change from a prolate to two spheres connected by a very narrow neck, indicating that the single long chain amphiphiles can be partitioned into the external monolayer in the lo phase of the GUV from the aqueous solution. As the single long chain amphiphile concentrations were increased, all of them induced vesicle fission of DPPC/chol(6/4) GUVs above their threshold concentrations. To elucidate the role of cholesterol in the single long chain amphiphile-induced vesicle fission, we investigated the effect of lyso-PC on GUVs of dioleoyl-PC (DOPC)/chol(6/4) membranes in the L-alpha phase; no vesicle fission occurred, indicating that cholesterol in itself did not play an important role in the vesicle fission. Finally, to elucidate the effect of the inclusion of DOPC in the lo-phase membrane of GUVs on the lyso-PC-induced vesicle fission of the DPPC/chol(6/4) GUV, we investigated the effect of low concentrations of lyso-PC on GUVs of DPPC/DOPC/chol membranes. With an increase in DOPC concentration in the membrane, the threshold concentration of lyso-PC increased. At and above 30 mol % DOPC, no vesicle fission occurred. On the basis of these results, we have proposed a hypothesis of the mechanism of the single long chain amphiphile-induced vesicle fission of a GUV of a lo-phase membrane.
机译：囊泡裂变是细胞中生物膜的非常重要的过程，但其机制尚不清楚，并存在争议。最近，我们使用单层巨型单层囊泡（GUV）方法发现，低浓度（低于临界胶束浓度（CMC））的溶血磷脂酰胆碱（lyso-PC）引起二棕榈酰磷脂酰胆碱/胆固醇（6/4）的GUV囊泡裂变（DPPC / chol（6/4））膜和鞘磷脂/胆固醇膜（6/4）处于液相有序（lo）相。在本报告中，为阐明其机理，我们研究了低浓度（远低于其CMC）具有单个长烃链的其他两亲物（即单个长链两亲物）对DPPC / chol（6/4）的影响GUV以及膜成分对溶血PC诱导的囊泡裂变的影响。我们发现低浓度的单个长链两亲物（溶血磷脂酸，辛基葡糖苷和十二烷基硫酸钠）会导致形状从扁长形变为两个由非常狭窄的颈部相连的球体，这表明单个长链两亲物可以被划分为水溶液中GUV的低相中有一个外部单层。随着单个长链两亲物浓度的增加，它们全部引起DPPC / chol（6/4）GUV的囊泡裂变，高于其阈值浓度。为了阐明胆固醇在单链长链两亲物诱导的囊泡裂变中的作用，我们研究了溶血-PC对L-α相中的油酰-PC（DOPC）/ chol（6/4）膜的GUV的影响。没有发生囊泡裂变，表明胆固醇本身在囊泡裂变中不发挥重要作用。最后，为阐明溶酶-PC诱导的DPPC / chol（6/4）溶酶-UV的囊泡裂变，在溶酶-PC诱导的囊泡裂变中掺入DOPC的影响，我们研究了低浓度溶酶-在DPPC / DOPC / chol膜的GUV上使用PC。随着膜中DOPC浓度的增加，溶血PC的阈值浓度增加。在大于或等于30mol％的DOPC时，没有发生囊泡裂变。基于这些结果，我们提出了单个长链两亲物诱导的lo-phase膜的GUV的囊泡裂变机制的假说。

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