Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy

Schon P; Gorlich M; Coenen MJJ; Heus HA; Speller S

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Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy

机译：原子力显微镜研究单分子水平的非特异性蛋白质吸附

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Liquid tapping atomic force microscopy was used to study the nonspecific adsorption of horse spleen ferritin at a bare gold surface at single molecule resolution. The majority of ferritin molecules adsorbed irreversible on gold surfaces in accordance with the random sequential adsorption (RSA) mechanism frequently used to describe irreversible adsorption processes. However, the time-resolved data also reveal events that go beyond the RSA model, i.e., lateral mobility and fragility of some molecules, resulting in desorption, chain formation, and subunit dissociation. Scanning effects of the AFM tip were observed, resulting in diminished protein coverage in the scanned area.

机译：液体攻丝原子力显微镜用于研究马脾铁蛋白在裸露的金表面上以单分子分辨率的非特异性吸附。根据经常用于描述不可逆吸附过程的随机顺序吸附（RSA）机制，大多数铁蛋白分子不可逆地吸附在金表面。但是，时间分辨数据还揭示了超出RSA模型的事件，即某些分子的横向迁移率和脆性，从而导致解吸，链形成和亚基解离。观察到了AFM尖端的扫描效果，导致扫描区域中蛋白质的覆盖率降低。

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《Langmuir: The ACS Journal of Surfaces and Colloids》 |2007年第20期|共3页
作者
Schon P; Gorlich M; Coenen MJJ; Heus HA; Speller S;
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正文语种 eng
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SURFACE-INDUCED AGGREGATION; HYDROPHOBIC SURFACE; FERRITIN ADSORPTION; KINETICS; INTERFACES; GOLD;

机译：表面诱导聚集;疏水表面;铁蛋白吸附;动力学;界面;金;

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1. Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy [J] . Schon P, Gorlich M, Coenen MJJ, Langmuir: The ACS Journal of Surfaces and Colloids . 2007,第20期

机译：原子力显微镜研究单分子水平的非特异性蛋白质吸附
2. Molecular Processes Studied at a Single-Molecule Level Using DNA Origami Nanostructures and Atomic Force Microscopy [J] . Bald Ilko, Keller Adrian Molecules . 2014,第9期

机译：使用DNA折纸纳米结构和原子力显微镜研究单分子水平的分子过程
3. Molecular Processes Studied at a Single-Molecule Level Using DNA Origami Nanostructures and Atomic Force Microscopy [J] . Adrian Keller, Ilko Bald Molecules . 2014,第9期

机译：使用DNA折纸纳米结构和原子力显微镜研究单分子水平的分子过程
4. The structure of Mn_6~(III) Cr~(III)~(3+) single-molecule magnets deposited in submonolayers and monolayers on surfaces studied by means of molecular resolved atomic force microscopy (AFM) and Kelvin Probe Force Microscopy in UHV [C] . U. Heinzmann, A. Gryzia, T. Volkmann, ICPEAC . 2014

机译：Mn_6〜（III）Cr〜（III）Cr〜（III）的结构〜（3+）单分子磁体沉积在通过分子分辨的原子力显微镜（AFM）和UHV中的Kelvin探针力显微镜（AFM）和Kelvin探针力显微镜研究的表面上的单层磁体
5. Adsorption behavior of proteins and colloidal particles studied using atomic force microscopy. [D] . Johnson, Christopher Allen. 1997

机译：使用原子力显微镜研究蛋白质和胶体颗粒的吸附行为。
6. Crystallographic order and decomposition of MnIII6CrIII3+ single-molecule magnets deposited in submonolayers and monolayers on HOPG studied by means of molecular resolved atomic force microscopy (AFM) and Kelvin probe force microscopy in UHV [O] . Aaron Gryzia, Timm Volkmann, Armin Brechling, 2014

机译：分子分辨原子力显微镜（AFM）和开尔文探针力显微镜研究了HOPG上亚单层和单层中MnIII6CrIII 3+单分子磁体的结晶顺序和分解
7. Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy [O] . Schon P., Gorlich M., Coenen M.J., 2007

机译：原子力显微镜研究单分子水平的非特异性蛋白质吸附

Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy

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