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首页> 外文期刊>Langmuir: The ACS Journal of Surfaces and Colloids >Facile construction of sulfanyl-terminated poly(ethylene glycol)-brushed layer on a gold surface for protein immobilization by the combined use of sulfanyl-ended telechelic and semitelechelic poly(ethylene glycol)s
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Facile construction of sulfanyl-terminated poly(ethylene glycol)-brushed layer on a gold surface for protein immobilization by the combined use of sulfanyl-ended telechelic and semitelechelic poly(ethylene glycol)s

机译：通过结合使用硫烷基末端的远螯和半遥远的聚乙二醇，在金表面上轻松构建以硫烷基为基的聚乙二醇刷层，以固定蛋白质

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摘要

A sulfanyl-terminated poly(ethylene glycol) (PEG)-brushed layer was constructed on a gold sensor platform by consecutive treatment with a sulfanyl-ended semitelechelic PEG (2 kDa, hereafter "MeO-PEG-SH (2k)") and a sulfanyl-ended telechelic PEG (5 kDa, hereafter "SH-PEG-SH (5k)"). Our strategy of constructing the sulfanyl-terminated PEG-brushed gold Surface is based on mixed-PEG-brush formation from the longer SH-PEG-SH (5k) and the shorter MeO-PEG-SH (2k), where the preimmobilized shorter MeO-PEG-SH (2k) prevents loop formation in the longer SH-PEG-SH (5k) on the surface and the free sulfanyl group at one end of the longer SH-PEG-SH is exposed to the mixed-PEG tethered-chain surface. From the experimental results obtained from surface plasmon resonance analysis, it became apparent that the immobilization density and the orientation of the longer SH-PEG-SH (5k) on the gold surface Could be controlled by the amount of preimmobilized shorter MeO-PEG-SH (2k). Under the optimized conditions of MeO-PEG-SH (2k) premodification, the constructed MeO-PEG-SH (2k)/SH-PEG-SH (5k) mixed layer conjugated efficiently with the maleimide-instal led proteins and the antibody Fab' fragments, accompanied by an appreciable nonfouling characteristic against bovine serum albumin as strong as that of the MeO-PEG-SH (5k)/MeO-PEG-SH (2k) mixed surface, which was reported in our previous work; it also showed a superior nonfouling characteristic compared to the commercially available carboxymethylated dextran surface (Uchida. K.; et al. Biointerphase 2007, 2 (4), 126-130). Furthermore, from the experimental results of the X-ray photoelectron spectrometry analysis, the presence of both Au-bound and Au-unbound sulfur species was confirmed on the SH-PEG-SH (5k)/MeO-PEG-SH (2k)-modified gold surface. These results clearly indicate that the preimmobilized shorter MeO-PEG-SH (2k) not only increased the nonfouling characteristic of the PEG tethered-chain surface but also prevented loop formation in the longer SH-PEG-SH (5k) on the gold surface. Since the protein-installed SH-PEG-SH (5k)/MeO-PEG-SH (2k)modified surface showed a strongly nonfouling characteristic and recognized the target molecules selectively, this new mixed-brush-formation technique using longer sulfanyl-ended telechelic PEGs and shorter semitelechelic PEGs is a simple yet effective method of constructing a strongly nonfouling terminal-functionalized gold surface for protein immobilization.
机译：通过用硫烷基末端的半遥远的PEG（2 kDa，以下称“ MeO-PEG-SH（2k）”）和亚甲酰基末端的半遥远的PEG连续处理，在金传感器平台上构建了以硫烷基为基的聚乙二醇（PEG）磨砂层。硫烷基末端的远螯PEG（5kDa，以下称为“ SH-PEG-SH（5k）”）。我们构建以硫烷基为基的PEG刷金表面的策略是基于较长的SH-PEG-SH（5k）和较短的MeO-PEG-SH（2k）（其中预先固定的较短MeO）的混合PEG-刷子形成-PEG-SH（2k）防止在较长的SH-PEG-SH（5k）表面形成环，且较长SH-PEG-SH一端的游离硫烷基暴露于混合PEG束缚链表面。从表面等离振子共振分析获得的实验结果，很明显，较长的SH-PEG-SH（5k）在金表面上的固定密度和取向可以通过预先固定的较短MeO-PEG-SH的量来控制（2k）。在MeO-PEG-SH（2k）预修饰的最佳条件下，构建的MeO-PEG-SH（2k）/ SH-PEG-SH（5k）混合层与马来酰亚胺-内嵌引导蛋白和抗体Fab'有效结合碎片，并具有与MeO-PEG-SH（5k）/ MeO-PEG-SH（2k）混合表面一样强的抗牛血清白蛋白的不结垢特性，这在我们先前的工作中已有报道;与市售的羧甲基化的葡聚糖表面相比，它还显示出优异的防污特性（Uchida.K .;等人，Biointerphase 2007，2（4），126-130）。此外，根据X射线光电子能谱分析的实验结果，在SH-PEG-SH（5k）/ MeO-PEG-SH（2k）-修饰的金表面。这些结果清楚地表明，预先固定的较短的MeO-PEG-SH（2k）不仅增加了PEG束缚链表面的防污特性，而且还防止了金表面较长的SH-PEG-SH（5k）中的环形成。由于安装了蛋白质的SH-PEG-SH（5k）/ MeO-PEG-SH（2k）修饰的表面显示出很强的防污特性并选择性地识别了目标分子，因此这种使用较长的亚硫酰基端远螯的混合刷形成技术PEG和较短的半遥远的PEG是一种简单而有效的方法，用于构建牢固的，不结垢的末端官能化金表面，以固定蛋白质。

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