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首页> 外文期刊>RSC Advances >An important prerequisite for efficient Forster resonance energy transfer (FRET) from human serum albumin to alkyl gallate
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An important prerequisite for efficient Forster resonance energy transfer (FRET) from human serum albumin to alkyl gallate

机译：从人血清白蛋白到没食子酸烷基酯有效进行Forster共振能量转移（FRET）的重要前提

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摘要

Forster resonance energy transfer (FRET), originally described by Forster in the 1940s, results from long-range dipole-dipole interactions between a donor and an acceptor molecule. In this work, human serum albumin (HSA) was selected as donor and the alkyl gallates were selected as acceptors. In the presence of HSA, the alkyl gallates yielded different levels of fluorescence enhancement. The corresponding energy transfer efficiency at a molar ratio of 1 : 1 were 0.7% (gallic acid), 0.2% (methyl gallate), 2.4% (ethyl gallate), 20.9% (propyl gallate), and 33.1% (butyl gallate). The alkyl gallates shared the same main binding site (located in Sudlow site II) in HSA. The formation of a stable complex by fixing alkyl gallate to tryptophan of HSA at an appropriate orientation and distance was an important prerequisite for efficient FRET. Mant factors, including the factors influencing the complex formation, can hinder energy transfer. Energy transfer was seriously hindered when fatty acids preexisted in HSA. During energy transfer, some energy were dissipated as heat. Energy transfer did not happen when the donor was egg white lysozyme rather than HSA. FRET does not exhibit specificity, but the specific structure of HSA helped provide the selectivity of alkyl gallate.
机译：福斯特（Forster）共振能量转移（FRET）最初是由福斯特（Forster）在1940年代描述的，其源于施主和受主分子之间的长距离偶极-偶极相互作用。在这项工作中，选择人血清白蛋白（HSA）作为供体，选择烷基没食子酸酯作为受体。在HSA存在下，没食子酸烷基酯产生不同水平的荧光增强。摩尔比为1：1时相应的能量转移效率为0.7％（没食子酸），0.2％（没食子酸甲酯），2.4％（没食子酸乙酯），20.9％（没食子酸丙酯）和33.1％（没食子酸丁酯）。没食子酸烷基酯在HSA中具有相同的主要结合位点（位于Sudlow位点II）。通过以适当的方向和距离将没食子酸烷基酯固定在HSA色氨酸上形成稳定的络合物是有效FRET的重要先决条件。许多因素，包括影响复合物形成的因素，都可能阻碍能量转移。 HSA中预先存在脂肪酸时，能量转移受到严重阻碍。在能量传递过程中，一些能量以热量的形式消散。当供体是蛋清溶菌酶而不是HSA时，能量转移没有发生。 FRET没有显示特异性，但是HSA的特定结构有助于提供没食子酸烷基酯的选择性。

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