Immobilization of dengue specific IgM antibodies on magnetite nanoparticles by using facile conjugation strategies

Ortega G. A.; Zuaznabar-Gardona J. C.; Morales-Tarre O.; Reguera E.

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Immobilization of dengue specific IgM antibodies on magnetite nanoparticles by using facile conjugation strategies

机译：使用简便的偶联策略将登革热特异性IgM抗体固定在磁铁矿纳米颗粒上

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IgM antibodies are the first immunoglobulin isotype to appear in several diseases like dengue fever. In this study, five different strategies to conjugate dengue specific IgM antibodies on magnetite nanoparticle surface (Fe3O4) were evaluated through commercial IgM antibody-capture ELISA assay for dengue diagnosis. IgM-Fe3O4 conjugates were obtained by using physical interactions, covalent bonding and antibody-antigen interactions. In all cases, analytical responses of IgM-Fe3O4 conjugates were higher than that of traditional ELISA assay. The most effective conjugation strategy was achieved by antigenantibody interaction with fluorescence intensity almost 20 times higher than standard ELISA. The results discussed herein illustrate the potential application of magnetite nanoparticles as a new platform for IgM immunoassay.

机译：IgM抗体是在登革热等几种疾病中出现的第一个免疫球蛋白同种型。在这项研究中，通过商用IgM抗体捕获ELISA分析评估了用于登革热诊断的五种不同策略，将登革热特异性IgM抗体偶联在磁铁矿纳米颗粒表面（Fe3O4）上。通过物理相互作用，共价键合和抗体-抗原相互作用获得IgM-Fe3O4缀合物。在所有情况下，IgM-Fe3O4偶联物的分析响应均高于传统ELISA分析。最有效的偶联策略是通过抗原抗体相互作用实现的，其荧光强度几乎是标准ELISA的20倍。本文讨论的结果说明了磁铁矿纳米颗粒作为IgM免疫测定新平台的潜在应用。

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《RSC Advances》 |2016年第100期|共9页
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Ortega G. A.; Zuaznabar-Gardona J. C.; Morales-Tarre O.; Reguera E.;
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1. Immobilization of dengue specific IgM antibodies on magnetite nanoparticles by using facile conjugation strategies [J] . Ortega G. A., Zuaznabar-Gardona J. C., Morales-Tarre O., RSC Advances . 2016,第100期

机译：使用简便的偶联策略将登革热特异性IgM抗体固定在磁铁矿纳米颗粒上
2. Site-Specific Antibody Conjugation Strategy to Functionalize Virus-Based Nanoparticles [J] . Park Jooneon, Chariou Paul L., Steinmetz Nicole F. Bioconjugate Chemistry . 2020,第5期

机译：特异性抗体缀合策略以官能化病毒的纳米颗粒
3. Presencia de anticuerpos IgM contra el virus del dengue en donantes de sangre, Medelln. Presence of IgM antibodies against dengue virus in blood donors, Medellin [J] . Berta Nelly Restrepo, Lina Marcela Arcila, Ruth Ramrez CES Medicina . 2010,第2期

机译：献血者中存在针对登革热病毒的IgM抗体，麦德林。献血者麦德林中存在针对登革热病毒的IgM抗体
4. Application of hydroxyapatite to detect specific antibodies -Anti-dengue virus IgM detection PA assay system approx [C] . Y. Kurosawa, A. Yamamoto, M. Nakayama Annual Meeting of The Ceramic Society of Japan . 2005

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5. Conjugation of Anti-HER2 Monoclonal Antibody onto a PLGA-PEG Nanoparticle Using CuAAC Click Chemistry [D] . Smith, Emily Alice 2012

机译：使用CuAAC单击化学将抗HER2单克隆抗体缀合到PLGA-PEG纳米颗粒上
6. Site-Specific Conjugation of Antibody on Gold Nanoparticle Surface for One-Step Diagnosis of Prostate Specific Antigen with Dynamic Light Scattering [O] . Nur Mustafaoglu, Tanyel Kiziltepe, Basar Bilgicer -1

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7. Interpretation of the presence of IgM and IgG antibodies in a rapid test for dengue: analysis of dengue antibody prevalence in Fortaleza City in the 20th year of the epidemic Interpretação da presença dos anticorpos IgM e IgG nos testes rápidos para dengue: análise da prevalência dos anticorpos da dengue em Fortaleza, no vigésimo ano da epidemia [O] . José Rubens Costa Lima, Maria Zélia Rouquayrol, Maria Roseli Monteiro Callado, 2012

机译：登革热流行20年中对登革热快速检测中IgM和IgG抗体的解释：登革热流行率分析登革热快速检测中对IgM和IgG抗体中存在的登革热的解释：抗体流行性分析流行第二十年在福塔雷萨的登革热
8. Mouse Monoclonal Antibody Specific for an Allotypic Determinant of the Igh(a) Allele of Murine IgM: Genetic and Functional Analysis of Igh-6a Epitopes Using Anti-IgM Monoclonal Antibodies. [R] . Sieckmann, D. G., Stall, A. M., Subbarao, B. 1991

机译：小鼠单克隆抗体特异性针对Igh（a）小鼠Igm等位基因的同种型决定因素：使用抗Igm单克隆抗体的Igh-6a表位的遗传和功能分析。

Immobilization of dengue specific IgM antibodies on magnetite nanoparticles by using facile conjugation strategies

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