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首页> 外文期刊>RSC Advances >Highly specific determination of gentamicin by induced collapse of Au-lipid capsules
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Highly specific determination of gentamicin by induced collapse of Au-lipid capsules

机译：通过金脂质胶囊的崩解高度特异性地测定庆大霉素

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摘要

Residues of gentamicin in food pose a threat to human health. Determining gentamicin residues relies largely on adequate analytical techniques. Herein, a simple and highly specific colorimetric method for effective detection of this aminoglycoside antibiotic in milk based on gentamicin-induced collapse of an Au-lipid capsule is first proposed. The strong interaction between gentamicin and phosphatidylcholine resulted in the collapse of Au-lipid capsule and consequently, the color of AuNPs changed from wine red to blue. The concentration of gentamicin could be determined with the naked eye or a UV-vis spectrometer. The results showed that the absorption ratio (A(664)/A(531)) was liner with the gentamicin concentration in the 0-0.2 mu M range with a linear 0.99 correlation coefficient. The detection limit was 7.4 nM. The coexisting substances, including L-arginine, guanidine hydrochloride, Tween-20, ammonium hydroxide, sodium chloride, potassium chloride, calcium chloride, glucose, and other common antibiotics such as streptomycin, amikacin, kanamycin, chloramphenicol, tetracycline, ampicillin and carbenicillin, did not interfere with the gentamicin determination with this method. Furthermore, the established method was successfully applied for the qualitative and quantitative analysis of gentamicin in pretreated milk products.
机译：食物中庆大霉素的残留物对人类健康构成威胁。庆大霉素残留的测定主要依靠适当的分析技术。在此，首先提出了一种简单且高度特异性的比色法，该方法基于庆大霉素诱导的金脂质胶囊的崩解有效检测牛奶中的氨基糖苷类抗生素。庆大霉素与磷脂酰胆碱之间的强相互作用导致金脂质胶囊的塌陷，因此金纳米颗粒的颜色从酒红色变为蓝色。庆大霉素的浓度可以用肉眼或紫外可见光谱仪测定。结果表明，庆大霉素浓度在0-0.2μM范围内，吸收比（A（664）/ A（531））呈线性关系，线性相关系数为0.99。检测限为7.4 nM。并存的物质包括L-精氨酸，盐酸胍，Tween-20，氢氧化铵，氯化钠，氯化钾，氯化钙，葡萄糖和其他常见抗生素，例如链霉素，阿米卡星，卡那霉素，氯霉素，四环素，氨苄青霉素和羧苄青霉素，不会干扰这种方法对庆大霉素的测定。此外，所建立的方法已成功地用于定乳产品中庆大霉素的定性和定量分析。

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