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首页> 外文期刊>RSC Advances >A novel strategy for low level laser-induced plasmonic photothermal therapy: the efficient bactericidal effect of biocompatible AuNPs@(PNIPAAM-co-PDMAEMA, PLGA and chitosan)
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A novel strategy for low level laser-induced plasmonic photothermal therapy: the efficient bactericidal effect of biocompatible AuNPs@(PNIPAAM-co-PDMAEMA, PLGA and chitosan)

机译：低水平激光诱导的等离子光热疗法的新策略：生物相容性AuNPs @（PNIPAAM-co-PDMAEMA，PLGA和壳聚糖）的有效杀菌作用

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Nowadays the control of multidrug resistant (MDR) and pandrug resistant (PDR) bacteria has been the subject of extensive research. In this study an effective strategy was developed to destroy bacteria using low-level laser photothermal therapy combined with biocompatible surface-modified AuNPs. First, chitosan, poly(N-isopropylacrylamide)-co-(2-dimethylamino ethyl methacrylate) P(NIPAAM-co-DMAEMA) and poly(lactic-coglycolic acid) (PLGA) modified AuNPs were synthesized and fully characterized. Afterwards, P. aeruginosa and A. baumannii (Two Gram-negative strains of bacteria) were exposed to different doses of low level NIR laser (810 nm) radiation in the presence or absence of the as-prepared surface-modified AuNPs (in a 1 : 1 ratio) and the killing efficiency of the radiated laser doses was calculated based on pour-plate colony count for each condition. Chitosan, P(NIPAAM-co-DMAEMA) and PLGA modified AuNPs were synthesized with a core/shell size of 108, 10 and 120 nm, respectively which was confirmed with DLS and TEM studies. Successful polymerization and surface coating of AuNPs was confirmed by FT-IR and H-1 NMR. A decreasing trend in the viability of both bacteria was observed along with an increase of the laser dose for all three types of polymer-coated AuNPs. PLGA@AuNPs exhibited the most effective NIR-induced photothermal killing on both bacteria. In other words, 10 J cm(-2) and 30 J cm(-2) doses were enough to destroy almost all P. aeruginosa, and A. baumannii, respectively. Our study suggests the usefulness of low-level laser in plasmonic photothermal treatment. The suggested strategy, as a new method of anti-bacterial intervention, can be used for the eradication of infections such as wound infections in order to accelerate the healing process. In addition, the offered strategy can be suggested in the treatment of other bio-threats such as cancerous diseases in vivo.
机译：如今，耐多药（MDR）和耐泛药（PDR）细菌的控制已成为广泛研究的主题。在这项研究中，开发了一种有效的策略，可以使用低水平激光光热疗法结合生物相容性表面修饰的AuNP来消灭细菌。首先，合成了壳聚糖，聚（N-异丙基丙烯酰胺）-共-（甲基丙烯酸2-二甲基氨基乙酯）P（NIPAAM-共-DMAEMA）和聚（乳酸-乙醇酸）（PLGA）修饰的AuNP。之后，在存在或不存在如此制备的表面修饰AuNP的情况下，将铜绿假单胞菌和鲍曼不动杆菌（两种革兰氏阴性菌）暴露于不同剂量的低水平NIR激光（810 nm）辐射下。比率为1：1），并根据每种情况下的倾板菌落计数，计算辐射激光剂量的杀灭效率。合成的壳聚糖，P（NIPAAM-co-DMAEMA）和PLGA修饰的AuNPs的核/壳尺寸分别为108、10和120 nm，这已通过DLS和TEM研究得到证实。 FT-IR和H-1 NMR证实了AuNPs的成功聚合和表面包覆。对于所有三种类型的聚合物包被的AuNP，观察到两种细菌的​​活力都有下降的趋势，并且激光剂量增加了。 PLGA @ AuNPs对两种细菌均表现出最有效的NIR诱导的光热杀死作用。换句话说，10 J cm（-2）和30 J cm（-2）的剂量足以破坏几乎所有铜绿假单胞菌和鲍曼不动杆菌。我们的研究表明低水平激光在等离子光热处理中的有用性。所建议的策略，作为一种新的抗菌干预方法，可用于根除伤口感染等感染，以加速愈合过程。另外，所提供的策略可以建议用于治疗其他生物威胁，例如体内癌性疾病。

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