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首页> 外文期刊>RSC Advances >Development of a dual screening strategy to identify pro-angiogenic compounds from natural products: application on Tongmai Yangxin Pills
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Development of a dual screening strategy to identify pro-angiogenic compounds from natural products: application on Tongmai Yangxin Pills

机译：建立双重筛选策略以从天然产物中鉴定促血管生成化合物：在通脉养心丸上的应用

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摘要

Therapeutic angiogenesis was regarded as a potential treatment for ischemic diseases, and natural products are invaluable resources to identify novel compounds with pro-angiogenic activities. However, reliable and rapid strategies to identify pro-angiogenic compounds from natural products with high-throughput screening still need to be developed. We first established a dual-screening strategy by integrating a proliferation assay of human umbilical vein endothelial cells (HUVECs) in vitro with an angiogenesis assay of Tg (Fli1 alpha: EGFP) transgenic zebra fishes in vivo to screen pro-angiogenic compounds. The active components from natural products were further identified via high performance liquid chromatography coupled with mass spectrometry. The proposed dual-screening strategy was applied to Tongmai Yangxin Pills (TMYXPs). Four active flavonoid compounds (glyasperin A, glycycoumarin, licorisoflavan A and licoisoflavone A) from TMYXPs were identified and were found for the first time to have satisfactory bioactivities for promoting HUVECs proliferation and angiogenesis in zebra fishes. Based on these results, this dual screening strategy was established to rapidly screen and identify bioactive components from natural products; this offers a novel screening assay for compounds isolated from complex natural products.
机译：治疗性血管生成被认为是缺血性疾病的潜在治疗方法，天然产物是鉴定具有促血管生成活性的新型化合物的宝贵资源。然而，仍需要开发可靠且快速的策略，以通过高通量筛选从天然产物中鉴定促血管生成化合物。我们首先通过将体外人脐静脉内皮细胞（HUVEC）的增殖测定与体内Tg（Fli1α：EGFP）转基因斑马鱼的血管生成测定相结合以筛选促血管生成化合物来建立双重筛选策略。通过高效液相色谱-质谱联用进一步鉴定天然产物的活性成分。拟议的双重筛选策略已应用于通脉养心丸（TMYXPs）。鉴定了来自TMYXPs的四种活性类黄酮化合物（甘油黄精A，糖香豆素，甘草异黄酮A和异黄酮A），并首次发现它们具有令人满意的生物活性，可促进斑马鱼HUVEC的增殖和血管生成。基于这些结果，建立了这种双重筛选策略以快速筛选和鉴定天然产物中的生物活性成分。这为从复杂天然产物中分离出的化合物提供了一种新颖的筛选方法。

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