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首页> 外文期刊>RSC Advances >Enzyme mediated biomass pretreatment and hydrolysis: a biotechnological venture towards bioethanol production
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Enzyme mediated biomass pretreatment and hydrolysis: a biotechnological venture towards bioethanol production

机译：酶介导的生物质预处理和水解：生物技术生产生物乙醇的尝试

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摘要

Biobased processes are gaining major interest worldwide with considerable efforts now being applied to developing efficient technologies for bioresource utilization. Enzymes produced from microorganisms act as a green route for lignocellulosic biomass pretreatment. Saacharum spontaneum or Kans grass is a potential lignocellulosic rich in cellulose (38.70%), hemicelluloses (29.00%), and lignin (17.46%). To utilize the major proportion of the carbohydrates such as cellulose and hemicelluloses to produce reducing sugar, degradation of lignin is an important prerequisite of the complete bioethanol production process. In the present work, an enzymatic pretreatment process for lignin degradation or delignification has been optimized through response surface methodology (RSM) based on central composite design (CCD). The maximum delignification recorded was 81.67% at 6 h upon monitoring the lignin content of 17.46%. The effectiveness of the enzymatic pretreatment process was investigated through various microscopic and spectroscopic tools as well through porosity analysis that evidenced the specific action of enzyme on lignin. Moreover, the efficacy of enzymatic pretreatment process on enzymatic hydrolysis was studied through optimization based on central composite design. The maximum reducing sugar obtained was 500.30 mg g(-1) at 5.30 h of incubation time which indeed supporting the potential of enzymatic mode of biomass pretreatment.
机译：生物基工艺在世界范围内引起了广泛的关注，目前已投入大量精力来开发有效的生物资源利用技术。由微生物产生的酶充当木质纤维素生物质预处理的绿色途径。 a草或堪萨斯草是一种潜在的木质纤维素，富含纤维素（38.70％），半纤维素（29.00％）和木质素（17.46％）。为了利用大部分碳水化合物，例如纤维素和半纤维素来产生还原糖，木质素的降解是完整生物乙醇生产过程的重要前提。在目前的工作中，木质素降解或去木质素的酶促预处理工艺已经通过基于中心复合设计（CCD）的响应面方法（RSM）进行了优化。监测木质素含量为17.46％，6小时记录的最大脱木质素率为81.67％。通过各种显微镜和分光镜工具以及通过孔隙率分析来研究酶预处理过程的有效性，孔隙率分析证明了酶对木质素的特殊作用。此外，通过基于中心复合设计的优化，研究了酶预处理工艺对酶水解的功效。在孵育时间5.30 h时获得的最大还原糖为500.30 mg g（-1），确实支持了生物质预处理的酶促模式。

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