• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Early release of 1-pyrroline by Pseudomonas aeruginosa cultures discovered using ambient corona discharge ionization mass spectrometry
【24h】

Early release of 1-pyrroline by Pseudomonas aeruginosa cultures discovered using ambient corona discharge ionization mass spectrometry

机译：使用环境电晕放电电离质谱法发现铜绿假单胞菌培养物可早期释放1-吡咯啉

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pseudomonas aeruginosa (PA) is a leading cause of nosocomial infections in humans with an increasing number of health-threatening implications, which urges faster clinical detection of this pathogen. In the present study, we discovered for the first time the early release of 1-pyrroline vapor by PA cultures using direct ambient mass spectrometry (AMS) analysis of the bacterial culture headspace based on corona discharge ionization. Importantly, the concentration of 1-pyrroline in PA cultures was found to greatly increase during the lag phase and early log phase of bacterial growth (3-6 h, 200-800 ppb), enabling early detection. Typically, 1-pyrroline produced by PA cultures could already be detected in our experiments after 0.5-4 h of incubation at the clinically relevant initial bacterial counts. A reference AMS screen of common infectious microbes from other genera, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Escherichia coli, Klebsiella pneumonia, Acinetobacter baumannii, Enterococcus faecalis, Klebsiella oxytoca, Candida albicans, Candida tropicalis, Candida parapsilosis, Enterococcus faecalis, Enterobacter cloacae, did not reveal a notable release of 1-pyrroline. Our results indicate the high suitability of volatile 1-pyrroline for the early and reliable diagnosis of Pseudomonas infections using commonly available MS instrumentation.
机译：铜绿假单胞菌（PA）是人类医院内感染的主要原因，对健康的威胁越来越大，这促使人们更快地对该病原体进行临床检测。在本研究中，我们首次使用基于电晕放电电离的细菌培养顶空直接环境质谱法（AMS）分析，通过PA培养首次发现了1-吡咯啉蒸气的早期释放。重要的是，发现PA培养物中1-pyrroline的浓度在细菌生长的滞后期和对数早期（3-6 h，200-800 ppb）期间大大增加，可以进行早期检测。通常，在临床相关的初始细菌数下孵育0.5-4小时后，在我们的实验中已经可以检测到PA培养物产生的1-吡咯啉。来自其他属的常见感染性微生物的参考AMS筛选，包括金黄色葡萄球菌，表皮葡萄球菌，溶血性葡萄球菌，大肠杆菌，鲍氏杆菌肺炎，鲍曼不动杆菌，粪肠球菌，副产肠杆菌，肠炎克雷伯氏菌，念珠菌，念珠菌泄殖腔没有显示1-吡咯啉的明显释放。我们的结果表明，使用常用的MS仪器，挥发性1-吡咯啉对假单胞菌感染的早期可靠诊断具有很高的适用性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号