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首页> 外文期刊>BioArchitecture >Tribolium embryo morphogenesis: May the force be with you
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Tribolium embryo morphogenesis: May the force be with you

机译：Tribolium胚胎形态发生：可能与您同在

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摘要

Development of multicellular organisms depends on patterning and growth mechanisms encoded in the genome, but also on the physical properties and mechanical interactions of the constituent cells that interpret these genetic cues. This fundamental biological problem requires integrated studies at multiple levels of biological organization: from genes, to cell behaviors, to tissue morphogenesis. We have recently combined functional genetics with live imaging approaches in embryos of the insect Tribolium castaneum, in order to understand their remarkable transformation from a uniform single-layered blastoderm into a condensed multi-layered embryo covered by extensive extra-embryonic tissues. We first developed a quick and reliable methodology to fluorescently label various cell components in entire Tribolium embryos. Live imaging of labeled embryos at single cell resolution provided detailed descriptions of cell behaviors and tissue movements during normal embryogenesis. We then compared cell and tissue dynamics between wild-type and genetically perturbed embryos that exhibited altered relative proportions of constituent tissues. This systematic comparison led to a qualitative model of the molecular, cellular and tissue interactions that orchestratethe observed epithelial rearrangements. We expect this work to establish the Tribolium embryo as a powerful and attractive model system for biologists and biophysicists interested in the molecular, cellular and mechanical control of tissue morphogenesis.
机译：多细胞生物的发展取决于基因组中编码的模式和生长机制，还取决于解释这些遗传线索的组成细胞的物理特性和机械相互作用。这个基本的生物学问题需要在多个生物学组织水平上进行综合研究：从基因到细胞行为再到组织形态发生。最近，我们将功能遗传学与活成像方法结合在了Tri虫Tribolium castaneum的胚胎中，以了解它们从均匀的单层胚盘到浓缩的多层胚的巨大转变，胚层被广泛的胚外组织覆盖。我们首先开发了一种快速可靠的方法来荧光标记整个Tribolium胚胎中的各种细胞成分。以单细胞分辨率对标记的胚胎进行实时成像提供了正常胚胎发生过程中细胞行为和组织运动的详细描述。然后，我们比较了野生型和遗传扰动的胚胎之间的细胞和组织动力学，这些胚胎表现出组成组织相对比例的变化。这种系统的比较导致了定性观察到的上皮重排的分子，细胞和组织相互作用的定性模型。我们希望这项工作能够将Tribolium胚胎确立为对感兴趣的组织形态发生的分子，细胞和机械控制感兴趣的生物学家和生物物理学家一个强大而有吸引力的模型系统。

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