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Full-field time-resolved fluorescence tomography of small animals

机译:小动物的全场时间分辨荧光层析成像

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摘要

In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo-based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model.
机译:在本实验研究中,我们探索了使用宽视野照明进行时间分辨荧光分子层析成像的可行性。在体外和小型动物模型中研究了具有时间分辨成像平台的宽视野模式的性能。基于时间选通的数据集,使用基于蒙特卡洛的正向模型重建荧光产量。在体外,与常规的cw数据类型相比,使用时间门数据类型时,分辨率和定量得到了改善。此外,通过精确定位植入鼠模型胸腔的荧光包裹体,可以确定用于荧光临床前应用的广域策略的可行性。

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