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Stereo vision in spatial-light-modulator-based microscopy

机译:基于空间光调制器的显微镜中的立体视觉

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We propose a technique for realizing stereoscopic microscopy. We employ a spatial-light-modulator-based microscope to record two images under different angles in one shot. We additionally investigate the possibilities of dynamic aberration correction. It is found that aberration correction is unavoidable because of the employed commercial liquid crystal on a silicon modulator. Also, imaging of phase objects and highly reflective specimens is experimentally investigated. For some of the specimens, an inversion of the recorded intensity is observed, which leads to problems when viewing the stereo pairs. We explain the origin of this effect and show that a reasonable visualization of microscopic three-dimensional objects can be achieved by simple image inversion.
机译:我们提出了一种实现立体显微镜的技术。我们使用基于空间光调制器的显微镜一次拍摄两个角度不同的图像。我们还研究了动态像差校正的可能性。已经发现,由于在硅调制器上使用了商用液晶,所以像差校正是不可避免的。此外,实验研究了相物和高反射标本的成像。对于某些样本,观察到记录强度的倒置,这在查看立体声对时会导致问题。我们解释了这种效应的起源,并表明可以通过简单的图像反演来实现微观三维物体的合理可视化。

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