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1064 nm dispersive Raman spectroscopy of tissues with strong near-infrared autofluorescence

机译:具有强近红外自发荧光的组织的1064 nm色散拉曼光谱

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摘要

Raman spectroscopy is an established technique for molecularly specific characterization of tissues. However, even with near-infrared (NIR) excitation, some tissues possess background autofluorescence, which can overwhelm Raman scattering. Here, we report collection of spectra from tissues with strong autofluorescence using a 1064 nm system with a high-throughput dispersive spectrometer and deep-cooled InGaAs array. Spectra collected at 1064 nm were compared with those collected at 785 nm in specimens from human breast, liver, and kidney. The results demonstrate superior performance at 1064 nm in the liver and kidney, where NIR autofluorescence is intense. The results indicate the feasibility of new biomedical applications for Raman spectroscopy at 1064 nm in tissues with strong autofluorescence.
机译:拉曼光谱法是用于组织的分子特异性表征的成熟技术。但是,即使使用近红外(NIR)激发,某些组织仍具有背景自发荧光,这可能使拉曼散射不堪重负。在这里,我们报告使用具有高通量色散光谱仪和深冷InGaAs阵列的1064 nm系统从具有强自发荧光的组织收集光谱。将在1064 nm处采集的光谱与在785 nm处采集的来自人类乳房,肝脏和肾脏的标本进行比较。结果表明,在NIR自发荧光强烈的肝脏和肾脏中,其在1064 nm处具有出色的性能。结果表明在具有强自发荧光的组织中用于1064 nm拉曼光谱的新生物医学应用的可行性。

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