...
首页> 外文期刊>Optics Letters >Sheet-scanned dual-axis confocal microscopy using Richardson-Lucy deconvolution
【24h】

Sheet-scanned dual-axis confocal microscopy using Richardson-Lucy deconvolution

机译:使用Richardson-Lucy反卷积的片扫描双轴共聚焦显微镜

获取原文
获取原文并翻译 | 示例
           

摘要

We have previously developed a line-scanned dual-axis confocal (LS-DAC) microscope with subcellular resolution suitable for high-frame-rate diagnostic imaging at shallow depths. Due to the loss of confocality along one dimension, the contrast (signal-to-background ratio) of a LS-DAC microscope is deteriorated compared to a point-scanned DAC microscope. However, by using a sCMOS camera for detection, a short oblique light-sheet is imaged at each scanned position. Therefore, by scanning the light sheet in only one dimension, a thin 3D volume is imaged. Both sequential two-dimensional deconvolution and three-dimensional deconvolution are performed on the thin image volume to improve the resolution and contrast of one enface confocal image section at the center of the volume, a technique we call sheet-scanned dual-axis confocal (SS-DAC) microscopy.
机译:我们以前已经开发了一种行扫描双轴共聚焦(LS-DAC)显微镜,该显微镜具有亚细胞分辨率,适用于浅深度的高帧率诊断成像。由于沿一维共焦损失,与点扫描DAC显微镜相比,LS-DAC显微镜的对比度(信噪比)降低。但是,通过使用sCMOS相机进行检测,可以在每个扫描位置成像短斜光片。因此,通过仅在一个维度上扫描光片,就可以对薄的3D体积成像。对薄图像体积执行顺序二维反褶积和三维反褶积,以提高位于体积中心的一个面共焦图像部分的分辨率和对比度,该技术称为片扫描双轴共焦(SS -DAC)显微镜检查。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号