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首页> 外文期刊>Optics Letters >Fast subcellular optical coherence photoacoustic microscopy for pigment cell imaging
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Fast subcellular optical coherence photoacoustic microscopy for pigment cell imaging

机译：快速亚细胞光学相干光声显微镜用于色素细胞成像

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摘要

We developed a fast ultrahigh resolution optical coherence photoacoustic microscopy (FU-OCPAM) system by combining two complementary imaging modes of optical coherence microscopy (OCM) and photoacoustic microscopy (PAM) for cellular/subcellular imaging. The system used optical scanning to realize fast imaging speed and provided ultrahigh resolution of 1.24 and 0.59 mu m for OCM and PAM, respectively. We imaged the retinal pigment epithelium (RPE) to demonstrate the subcellular imaging capability of the FU-OCPAM system. The OCM and PAM images clearly showed the RPE cell morphology and reflected the complementary optical properties of scattering and absorption. A quantitative analysis of the RPE cells was made based on photoacoustic (PA) signals. The cell area mainly ranged from 80 to 300 mu m(2), and had a linear relationship with the sum intensity of PA signals which mainly reflected the melanin content of the cells. Themorphology and the PA signal could be used to identify qualitatively and quantitatively the aging and healthy states of the RPE cells. The results show the potential applications in studying the real-time cellular response to external stimulations and the progress of aging and diseases at the cellular level with FU-OCPAM. (C) 2015 Optical Society of America
机译：我们通过结合光学相干显微镜（OCM）和光声显微镜（PAM）的两种互补成像模式进行细胞/亚细胞成像，开发了快速超高分辨率光学相干光声显微镜（FU-OCPAM）系统。该系统使用光学扫描实现快速成像，并为OCM和PAM分别提供了1.24和0.59μm的超高分辨率。我们对视网膜色素上皮（RPE）进行了成像，以证明FU-OCPAM系统的亚细胞成像能力。 OCM和PAM图像清楚地显示了RPE细胞的形态，并反映了散射和吸收的互补光学特性。根据光声（PA）信号对RPE细胞进行定量分析。细胞面积主要在80至300μm（2）之间，与PA信号总和强度呈线性关系，PA信号的总强度主要反映细胞黑色素的含量。形态学和PA信号可用于定性和定量鉴定RPE细胞的衰老和健康状态。结果表明，使用FU-OCPAM可以实时研究细胞对外部刺激的实时反应以及衰老和疾病进程。 （C）2015年美国眼镜学会

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