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Hyperspectral time-resolved wide-field fluorescence molecular tomography based on structured light and single-pixel detection

机译:基于结构光和单像素检测的高光谱时间分辨宽场荧光分子层析成像

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摘要

We present a time-resolved fluorescence diffuse optical tomography platform that is based on wide-field structured illumination, single-pixel detection, and hyperspectral acquisition. Two spatial light modulators (digital micro-mirror devices) are employed to generate independently wide-field illumination and detection patterns, coupled with a 16-channel spectrophotometer detection module to capture hyperspectral time-resolved tomographic data sets. The main system characteristics are reported, and we demonstrate the feasibility of acquiring dense 4D tomographic data sets (space, time, spectra) for time domain 3D quantitative multiplexed fluorophore concentration mapping in turbid media. (C) 2015 Optical Society of America
机译:我们提出了一个基于宽域结构照明,单像素检测和高光谱采集的时间分辨荧光漫射光学层析成像平台。两个空间光调制器(数字微镜设备)用于生成独立的宽视野照明和检测图案,并与16通道分光光度计检测模块耦合以捕获高光谱时间分辨断层扫描数据集。报告了主要的系统特征,并且我们证明了获取稠密的4D层析数据集(空间,时间,光谱)以进行混浊介质中时域3D定量多路复用荧光团浓度映射的可行性。 (C)2015年美国眼镜学会

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