Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416 x 832 x 160 mu m field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain. (C) 2016 Optical Society of America
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机译：增加光片显微镜的体积成像速度将提高其检测神经活动快速变化的能力。在这里，介绍了一种系统，该系统通过将结构照明与立方相扩展景深（EF）瞳孔编码耦合，对幼虫斑马鱼的神经活动进行全脑成像。该显微镜可实现更快的光片成像，并有利于任意平面扫描，消除了对采集速度，对准公差和样品附近物理运动的限制。该方法的有用性通过在鱼脑中以416 x 832 x 160微米（33 Hz）的视场执行多平面钙成像来证明。斑马鱼的光动力反应行为被高速监测，并且神经元活动的时间相关性在其大脑中得以分解。 （C）2016美国眼镜学会
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