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High-dynamic-range microscope imaging based on exposure bracketing in full-field optical coherence tomography

机译:基于全场光学相干断层扫描曝光包围的高动态范围显微镜成像

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摘要

By applying the proposed high-dynamic-range (HDR) technique based on exposure bracketing, we demonstrate a meaningful reduction in the spatial noise in image frames acquired with a CCD camera so as to improve the fringe contrast in full-field optical coherence tomography (FF-OCT). This new signal processing method thus allows improved probing within transparent or semitransparent samples. The proposed method is demonstrated on 3 mu m thick transparent polymer films of Mylar, which, due to their transparency, produce low contrast fringe patterns in white-light interference microscopy. High-resolution tomographic analysis is performed using the technique. After performing appropriate signal processing, resulting XZ sections are observed. Submicrometer-sized defects can be lost in the noise that is present in the CCD images. With the proposed method, we show that by increasing the signal-to-noise ratio of the images, submicrometer-sized defect structures can thus be detected. (C) 2016 Optical Society of America
机译:通过应用基于曝光包围的提议的高动态范围(HDR)技术,我们证明了CCD相机采集的图像帧中空间噪声的有效降低,从而改善了全场光学相干断层扫描的条纹对比度( FF-OCT)。因此,这种新的信号处理方法可以改进透明或半透明样本内的探测。该方法在聚酯薄膜的3微米厚的透明聚合物薄膜上得到了证明,由于其透明性,在白光干涉显微镜下可产生低对比度的条纹图案。使用该技术进行高分辨率的层析成像分析。执行适当的信号处理后,将观察所得的XZ部分。 CCD图像中存在的噪声可能会损失亚微米级的缺陷。使用所提出的方法,我们表明通过增加图像的信噪比,可以检测出亚微米级的缺陷结构。 (C)2016美国眼镜学会

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