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Real-time quantitative phase imaging based on transport of intensity equation with dual simultaneously recorded field of view

机译:基于同时传输双视场的强度方程传输的实时定量相位成像

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摘要

Since quantitative phase distribution reflects both cellular shapes and conditions from another view, compared to traditional intensity observation, different quantitative phase microscopic methods are proposed for cellular detections. However, the transport of intensity equation-based approach not only presents phase, but also intensity, which attracts much attention. While classical transport of intensity equation needs multi-focal images which often cannot realize simultaneous phase measurement, in this Letter, to break through the limitation, a real-time quantitative phase imaging method using transport of intensity equation is proposed. Two identical CCD cameras are set at the binocular tubes to capture the same field of view but at different focal planes. With a double-frame algorithm assuming that the on-focal image is the average of over-and under-focal information, the proposed method is capable of calculating quantitative phase distributions of samples accurately and simultaneously indicating its potentialities in cellular real-time monitoring. (C) 2016 Optical Society of America
机译:由于定量相分布从另一个角度反映了细胞的形状和条件,因此与传统的强度观察相比,提出了不同的定量相显微镜方法进行细胞检测。然而,基于强度方程的方法的传递不仅呈现相位,而且呈现强度,这引起了很多关注。虽然经典的强度方程式输运需要多焦点图像,而这些图像通常无法实现同时的相位测量,但在本信中,为了突破局限性,提出了一种使用强度方程式传递的实时定量相位成像方法。在双筒镜筒上设置了两个相同的CCD摄像机,以捕获相同的视野,但在不同的焦平面上。利用双帧算法,假设焦点上的图像是焦点上和焦点下信息的平均值,则该方法能够准确计算样本的定量相位分布,并同时表明其在细胞实时监测中的潜力。 (C)2016美国眼镜学会

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