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首页> 外文期刊>Physical chemistry chemical physics: PCCP >Influence of the linker type on the Au-S binding properties of thiol and disulfide-modified DNA self-assembly on polycrystalline gold
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Influence of the linker type on the Au-S binding properties of thiol and disulfide-modified DNA self-assembly on polycrystalline gold

机译：接头类型对多晶金上硫醇和二硫键修饰的DNA自组装的Au-S结合特性的影响

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摘要

We investigate the Au-S binding properties of thiol and disulfide-modified DNA on polycrystalline gold by means of X-ray photoelectron spectroscopy in conditions close to dynamic processes of biosensors. The dependence of the immobilisation period on the quality and density of the self-assembly process of thiol (SH-(CH2)6-DNA), disulfide (DNA-CH2)6-SS-(CH2)6-DNA and DMTO-SS-(CH2)6-DNA) sulfur-modified oligonucleotide solutions (1 μM) that are employed for bioreceptor immobilisation is analysed. Two electronic components are found in the analysis of the S 2p core levels. One of them is clearly associated to thiolate formation, while the other can be associated to different origins. In order to identify the origin of this last component, a quantification of the non-specifically adsorbed species has been performed by rinsing the self-assembled monolayers (SAMs) with a mercapto hexanol (MCH) solution. It has been found that non-specifically adsorbed species contribute only partially to the appearance of this sulfur peak component in SAMs formed from disulfides. Electron bombardment was performed to study the evolution of this component as a consequence of surface degradation due to radiation effects. The results are also correlated with the possible presence of disulfides. We found that MCH is not stable during the measurements. The evolution of this compound and the possible causes for this behaviour are discussed.
机译：我们在接近生物传感器动态过程的条件下，通过X射线光电子能谱研究了硫醇和二硫键修饰的DNA在多晶金上的Au-S结合特性。固定期对硫醇（SH-（CH2）6-DNA），二硫键（DNA-CH2）6-SS-（CH2）6-DNA和DMTO-SS自组装过程的质量和密度的依赖性分析了用于生物受体固定化的-（CH2）6-DNA）硫修饰寡核苷酸溶液（1μM）。在分析S 2p核心级别时发现了两个电子组件。其中一个显然与硫醇盐的形成有关，而另一个可能与不同的来源有关。为了鉴定该最后一种成分的来源，已通过用巯基己醇（MCH）溶液冲洗自组装单层（SAM）进行了非特异性吸附物质的定量。已经发现，非特异性吸附的物质仅部分促成由二硫化物形成的SAM中该硫峰组分的出现。进行电子轰击以研究由于辐射效应导致的表面退化导致该成分的演变。结果也与可能存在的二硫化物相关。我们发现在测量过程中MCH不稳定。讨论了该化合物的演变以及此行为的可能原因。

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