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首页> 外文期刊>Biochimica et biophysica acta. Molecular basis of disease: BBA >Characterization of an 84 kDa protein inducing an immediate hypersensitivity reaction in rabbits sensitized to Haemaphysalis longicornis ticks
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Characterization of an 84 kDa protein inducing an immediate hypersensitivity reaction in rabbits sensitized to Haemaphysalis longicornis ticks

机译：表征84 kDa的蛋白，可引起对Haemaphysalis longicornis壁虱致敏的兔子立即超敏反应

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摘要

An immunogenic 84 kDa protein was isolated and purified from whole tick extracts of Haemaphysalis longicornis larvae by a combination of ion exchange, reverse phase and hydrophobic interaction chromatographies. The protein, when injected intradermally into rabbits exposed to repeated tick feeding, induces an immediate cutaneous hypersensitivity reaction. It has been purified to homogeneity as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis and silver staining. Amino acid sequences for two peptides derived from proteolytic cleavage of p84 were scanned against known proteins on the SWISS-PROT database. A 7 residue motif, ISGWGNT present in one of the two peptides appeared conserved in both vertebrate and invertebrate trypsin-like serine proteinases, while another 7 amino acid motif, HVPAGQI present in the second peptide showed homology to an Escherichia coli ATP-binding protein. We have discussed our findings in relation to isolation and characterization of target antigens for tick vaccine candidates.
机译：通过离子交换色谱，反相色谱和疏水相互作用色谱的结合，从长血ema幼虫的全壁虱提取物中分离并纯化了具有免疫原性的84 kDa蛋白。当将这种蛋白质皮内注射到反复repeated饲的兔子体内时，会立即引起皮肤超敏反应。如十二烷基硫酸钠聚丙烯酰胺凝胶电泳和银染所示，已纯化至均一。在SWISS-PROT数据库中针对已知蛋白扫描了源自p84蛋白水解切割的两个肽的氨基酸序列。存在于两个肽之一中的7个残基基序ISGWGNT在脊椎动物和无脊椎动物胰蛋白酶样丝氨酸蛋白酶中均是保守的，而存在于第二个肽中的另外7个氨基酸基序HVPAGQI与大肠杆菌ATP结合蛋白具有同源性。我们已经讨论了有关tick疫苗候选靶抗原的分离和表征的发现。

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