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首页> 外文期刊>Plant physiology >RBF1, a Plant Homolog of the Bacterial Ribosome-Binding Factor RbfA, Acts in Processing of the Chloroplast 16S Ribosomal RNA
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RBF1, a Plant Homolog of the Bacterial Ribosome-Binding Factor RbfA, Acts in Processing of the Chloroplast 16S Ribosomal RNA

机译：RBF1，细菌核糖体结合因子RbfA的植物同源物，在叶绿体16S核糖体RNA的加工中起作用。

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摘要

Plastids (chloroplasts) possess 70S ribosomes that are very similar in structure and function to the ribosomes of their bacterial ancestors. While most components of the bacterial ribosome (ribosomal RNAs [rRNAs] and ribosomal proteins) are well conserved in the plastid ribosome, little is known about the factors mediating the biogenesis of plastid ribosomes. Here, we have investigated a putative homolog of the bacterial RbfA (for ribosome-binding factor A) protein that was identified as a coldshock protein and an auxiliary factor acting in the 59 maturation of the 16S rRNA. The unicellular green alga Chlamydomonas reinhardtii and the vascular plant Arabidopsis (Arabidopsis thaliana) both encode a single RbfA-like protein in their nuclear genomes. By generating specific antibodies against this protein, we show that the plant RbfA-like protein functions exclusively in the plastid, where it is associated with thylakoid membranes. Analysis of mutants for the corresponding gene (termed RBF1) reveals that the gene function is essential for photoautotrophic growth. Weak mutant alleles display reduced levels of plastid ribosomes, a specific depletion in 30S ribosomal subunits, and reduced activity of plastid protein biosynthesis. Our data suggest that, while the function in ribosome maturation and 16S rRNA 59 end processing is conserved, the RBF1 protein has assumed an additional role in 39 end processing. Together with the apparent absence of a homologous protein from plant mitochondria, our findings illustrate that the assembly process of the 70S ribosome is not strictly conserved and has undergone some modifications during organelle evolution.
机译：质体（叶绿体）拥有70S核糖体，其结构和功能与其细菌祖先的核糖体非常相似。虽然细菌核糖体的大多数成分（核糖体RNA [rRNAs]和核糖体蛋白）在质体核糖体中保存良好，但对介导质体核糖体生物发生的因素知之甚少。在这里，我们调查了细菌RbfA（对于核糖体结合因子A）蛋白的推定同源物，该蛋白被鉴定为冷休克蛋白和在16S rRNA成熟59中起作用的辅助因子。单细胞绿藻莱茵衣藻和维管植物拟南芥（Arabidopsis thaliana）在它们的核基因组中都编码单个RbfA样蛋白。通过生成针对这种蛋白质的特异性抗体，我们显示出植物RbfA样蛋白质仅在质体中起作用，在该质体中与类囊体膜相关。对相应基因（称为RBF1）的突变体进行分析后发现，该基因功能对于光合自养生长至关重要。弱的突变等位基因显示出降低的质体核糖体水平，30S核糖体亚基的特定消耗以及质体蛋白生物合成的活性降低。我们的数据表明，虽然在核糖体成熟和16S rRNA 59末端加工中的功能得以保留，但RBF1蛋白在39末端加工中承担了额外的角色。我们的发现与植物线粒体中同源蛋白的明显缺失一起表明，70S核糖体的组装过程并非严格保守，在细胞器进化过程中发生了一些修饰。

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