• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cornea >Acidic mammalian chitinase in dry eye conditions.
【24h】

Acidic mammalian chitinase in dry eye conditions.

机译：在干眼条件下为酸性哺乳动物几丁质酶。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

PURPOSE: An acidic mammalian chitinase (AMCase) seems to be implicated in allergic asthma and allergic ocular pathologies. The aim of this work was to investigate the role of AMCase during Sjogren's Syndrome (SS) and Meibomian Gland Dysfunction (MGD) dry eye diseases. METHODS: Six patients with MGD dry eye (20-58 years, median 40) and six patients with dry eye associated to SS (32-60 years, median 47) were enrolled in this study. AMCase activity was measured in tears and AMCase mRNA expression was evaluated by real-time polymerase chain reaction from RNA extracted from epithelial cells of the conjunctiva. Six healthy adult subjects of the same age (34-44 years, median 39) were also studied as the control group. RESULTS: AMCase activity was significantly increased in patients affected by MGD dry eye (18.54 +/- 1.5 nmol/ml/h) and SS dry eye (8.94 +/- 1.0 nmol/ml/h) respectively, compared to healthy controls (1.6 +/- 0.2 nmol/ml/h). AMCase activity was higher in the tears of subjects with MGD dry eye (P < 0.001). AMCase mRNA was detected in conjunctival epithelial cells and the expression was significantly higher in MGD dry eye than SS dry eye. A significant correlation between AMCase activity in the tears and mRNA in conjunctival epithelial cells was found. CONCLUSION: AMCase may be an important marker in the pathogenesis of dry eye, suggesting the potential role of AMCase as a therapeutic target in these frequent pathologies.
机译：目的：酸性哺乳动物几丁质酶（AMCase）似乎与过敏性哮喘和过敏性眼病有关。这项工作的目的是调查AMCase在干燥综合征（SS）和睑板腺功能障碍（MGD）干眼症中的作用。方法：本研究招募了6例MGD干眼患者（20-58岁，中位40岁）和6例与SS相关的干眼患者（32-60岁，中位47岁）。测量眼泪中的AMCase活性，并通过实时聚合酶链反应，从结膜上皮细胞提取的RNA中评估AMCase mRNA的表达。还研究了六个相同年龄（34-44岁，中位数39岁）的健康成人受试者作为对照组。结果：与健康对照组（1.6）相比，受MGD干眼（18.54 +/- 1.5 nmol / ml / h）和SS干眼（8.94 +/- 1.0 nmol / ml / h）影响的患者AMCase活性显着增加+/- 0.2 nmol / ml / h）。 MGD干眼受试者的眼泪中AMCase活性较高（P <0.001）。在结膜上皮细胞中检测到AMCase mRNA，并且MGD干眼中的表达明显高于SS干眼。发现眼泪中的AMCase活性与结膜上皮细胞的mRNA之间存在显着相关性。结论：AMCase可能是干眼病发病机制中的重要标志物，提示AMCase作为治疗靶点在这些常见病理中的潜在作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号