• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cornea >Increased expression of fibrillin-1 in human corneas with bullous keratopathy.
【24h】

Increased expression of fibrillin-1 in human corneas with bullous keratopathy.

机译：fibrillin-1在大疱性角膜病变的人角膜中的表达增加。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

PURPOSE: To characterize the expression of fibrillins, microfibril components, in human corneas with pseudophakic/aphakic (PBK/ABK) bullous keratopathy. METHODS: Normal and PBK/ABK corneas were stained by immunofluorescence for fibrillin-1 and -2. The expression of fibrillin-1 messenger RNA (mRNA) was studied by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and Southern analysis. RESULTS: Only fibrillin-1 was detected in normal and diseased corneas. As described previously, in normal corneas, it was found in the limbal stroma and basement membrane (BM) and in the peripheral corneal epithelial BM for a short distance near the limbus. Central corneal BM, stroma, and Descemet's membrane were negative. All PBK/ABK corneas were positive for fibrillin-1, which was detected in fibrillar deposits at the endothelial face of Descemet's membrane, in the epithelial BM, subepithelial fibrosis areas, and posterior collagenous layer. By RT-PCR, low levels of fibrillin-1 mRNA were detected in normal corneas, and they increased significantly in PBK/ABK corneas. CONCLUSION: The deposition of fibrillin-1, together with tenascin-C, in PBK/ABK corneas may be part of an abnormal fibrotic/wound-healing process that occurs during the development of postsurgical corneal edema with the formation of bullae and posterior collagenous layer.
机译：目的：表征原发性/无晶状体（PBK / ABK）大疱性角膜病变的人角膜中原纤维蛋白，微纤维成分的表达。方法：通过免疫荧光法对原纤维和PBK / ABK角膜进行原纤维蛋白-1和-2染色。通过半定量逆转录-聚合酶链反应（RT-PCR）和Southern分析研究了fibrillin-1信使RNA（mRNA）的表达。结果：在正常和患病的角膜中仅检出了fibrillin-1。如前所述，在正常角膜中，在角膜缘附近的短距离内，在角膜基质和基底膜（BM）和周围角膜上皮BM中发现了它。中央角膜BM，基质和Descemet膜阴性。所有PBK / ABK角膜均检测到原纤维蛋白1阳性，该蛋白在Descemet膜的内皮表面，上皮BM，上皮下纤维化区域和后胶原层的原纤维沉积物中检测到。通过RT-PCR，在正常角膜中检测到低水平的原纤维蛋白-1 mRNA，在PBK / ABK角膜中它们显着增加。结论：原纤维蛋白-1和腱糖蛋白C沉积在PBK / ABK角膜中可能是异常纤维化/伤口愈合过程的一部分，该过程在术后角膜水肿的发展过程中发生，形成大疱和后胶原层。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号