• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cornea >Descemet-stripping automated endothelial keratoplasty: effect of anterior lamellar corneal tissue-on/-off storage condition on Descemet-stripping automated endothelial keratoplasty donor tissue.
【24h】

Descemet-stripping automated endothelial keratoplasty: effect of anterior lamellar corneal tissue-on/-off storage condition on Descemet-stripping automated endothelial keratoplasty donor tissue.

机译：Descemet-stripping内皮自动角膜移植术：前层状角膜组织的开/关存储条件对Descemet-stripping内皮自动角膜移植供体组织的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

PURPOSE: To compare the effect of storing Descemet-stripping automated endothelial keratoplasty (DSAEK) tissue with anterior lamellar corneal tissue (ALCT)-on versus -off. METHODS: An in vitro model was used with corneoscleral rims and DSAEK quality paired corneas. After microkeratome-assisted excision of ALCT, 4 pairs of corneas (8 eyes) were stored with the ALCT left on the stroma (on) and the others with ALCT off the stroma (off) for 24 hours in Optisol GS solution. A vital dye assay was used to identify devitalized and necrotic endothelial cells with alizarin red S and trypan blue. RESULTS: Corneal endothelial cell damage was observed in the ALCT-off specimens, whereas almost no staining was observed in the ALCT-on samples. In addition, the ALCT-off donor corneas were clinically edematous and opaque, whereas the ALCT-on corneas were clear. Moreover, Descemet membranes of ALCT-off samples were found to be loose and easily detached from the stroma, with many Descemet striae observed in the specimens.CONCLUSIONS: This study shows that endothelial damage occurs in ALCT-off corneas. We hypothesize that the absence of the Bowman layer may contribute to the damage, because it has been shown that the Bowman layer provides a barrier function. These data suggest that it is important to keep the ALCT/Bowman layer on the stromal side of the DSAEK graft as long as possible to avoid stromal swelling and endothelial damage.
机译：目的：比较将带Descemet剥离的自动内皮角膜移植术（DSAEK）组织与前板层角膜组织（ALCT）的开-关状态进行存储的效果。方法：采用角膜巩膜边缘和DSAEK优质成对角膜的体外模型。在微角膜刀辅助下切除ALCT后，将4对角膜（8眼）在Optisol GS溶液中保存，将ALCT留在基质上（打开），将ALCT留在基质上（关闭），放置24小时。生命染料测定法用于鉴定具有茜素红S和锥虫蓝的失活和坏死的内皮细胞。结果：在ALCT-off标本中观察到角膜内皮细胞损伤，而在ALCT-on标本中几乎未观察到染色。此外，ALCT脱落的供体角膜在临床上是水肿和不透明的，而ALCT脱落的供体角膜是透明的。此外，ALCT-off样品的Descemet膜很松散，很容易从基质上脱落，在标本中观察到许多Descemet纹。结论：这项研究表明，ALCT-off角膜发生了内皮损伤。我们假设缺少Bowman层可能会造成损坏，因为已经证明Bowman层提供了屏障功能。这些数据表明，将ALCT /鲍曼层保持在DSAEK移植物基质侧的时间尽可能长很重要，以避免基质溶胀和内皮损伤。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号