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In vitro propagation studies in cardamom.

机译：豆蔻的体外繁殖研究。

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摘要

Tissue culture technology is used for selection and rapid multiplication of elite clones of cardamom. In India, the micro propagation technique has gained momentum to reoccupy the monopoly in cardamom production at global level. It is able to regenerate millions of copies to ensure in a decade of time with high yielding and disease free material. The present study was undertaken by using three varieties, namely, ICRI 2, TDK 4 and GG. Different explants of cardamom viz., shoot bud, shoot tip, rhizome and seed were inoculated in MS medium (Murashige and Skoog, 1962) supplemented with BAP, Ca pantothenete, IAA, 2,4-D and coconut water. The growth of multiple shoots was more vigorous on MS medium with BAP (1.50 mg/l) and Ca pantothenate 0.4 mg/l. The variety TDK 4 had good direct organogenesis reports compared to other varieties. The explant shoot bud had good efficiency for induction of callus in TDK 4 variety.
机译：组织培养技术用于选择和快速繁殖豆蔻的优良克隆。在印度，微繁殖技术获得了动力，重新占据了全球豆蔻果实生产的垄断地位。它能够再生数百万份副本，以确保在十年内获得高产量且无病的材料。本研究通过使用三个品种进行，即ICRI 2，TDK 4和GG。将小豆蔻的不同外植体，芽，茎尖，根茎和种子接种在补充有BAP，泛酸钙，IAA，2,4-D和椰子水的MS培养基中（Murashige和Skoog，1962）。在BAP（1.50 mg / l）和泛酸Ca 0.4mg / l的MS培养基上，多芽的生长更加旺盛。与其他品种相比，TDK 4品种具有良好的直接器官发生报告。外植芽对TDK 4品种愈伤组织有良好的诱导作用。

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