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Impact of mutagenesis on physiological and biochemical parameters of coleus (Coleus forskohlii Briq.).

机译:诱变对锦鲤( Coleus forskohlii Briq。)的生理和生化指标的影响。

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The investigation on induced mutagenesis on physiological and biochemical parameters of coleus was carried out at Medicinal Plant Unit, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore using the terminal cuttings of coleus ecotype 'Garmai'. The results revealed that the maximum crop growth rate (5.04 g/m2/day) was observed in 0.50% EMS. While the minimum crop growth rate (3.10 g/m2/day) was recorded at higher doses of mutagen (3.50 kR gamma rays+1.50% EMS). The maximum (28.50) and minimum (26.09) harvest index was recorded in 2.50 kR gamma rays+1.00% EMS and 3.50 kR gamma rays, respectively. The mean total dry matter production of different mutagenic treatments reduced progressively from the control (181.89 g) to (3.50 kR gamma rays+1.50% EMS (97.31 g)). The mean total carbohydrate content ranged from 12.08 mg glucose/g material (3.50 kR gamma rays+1.00% EMS) to 27.90 mg glucose/g material (control). The mean soluble protein content ranged from 5.96 mg albumin equivalent to soluble protein/g (3.50 kR gamma rays+1.50% EMS) to 8.90 mg albumin equivalent to soluble protein/g (control). The mutagenic treatments recorded reduced mean values over control for IAA oxidase activity in leaves. The decrease in mean values was consistent at higher doses of mutagen. The mean values for peroxidase activity decreased with an increasing dose of mutagens except in 0.50% EMS (3.91 change in OD at 430 nm/min/g). All theses parameters contribute the low fresh tuber yield with consecutive increase in mutagenic doses.
机译:在哥伦布托的泰米尔纳德邦农业大学园艺学院和研究所的药用植物部门,使用了锦葵属生态型“ Garmai”的末端插条,对锦鲤的生理和生化参数进行了诱变研究。结果表明,在0.50%的环境管理体系中,最大农作物生长速率为5.04 g / m 2 /天。在更高剂量的诱变剂(3.50 kR伽马射线+ 1.50%EMS)下记录了最低作物生长速度(3.10 g / m 2 /天)。最高收获指数(28.50)和最低收获指数(26.09)分别记录在2.50 kRγ射线+ 1.00%EMS和3.50 kRγ射线中。不同诱变处理的平均总干物质产量从对照(181.89 g)逐渐降低至(3.50 kRγ射线+ 1.50%EMS(97.31 g))。平均总碳水化合物含量范围为12.08 mg葡萄糖/ g材料(3.50 kR伽马射线+ 1.00%EMS)至27.90 mg葡萄糖/ g材料(对照)。平均可溶性蛋白含量的范围是:5.96 mg白蛋白当量/g(3.50 kR伽马射线+ 1.50%EMS)至8.90 mg白蛋白当量/ g(对照)。诱变处理记录的叶片中IAA氧化酶活性的平均值低于对照。在较高剂量的诱变剂下,平均值的下降是一致的。过氧化物酶活性的平均值随诱变剂剂量的增加而降低,但在0.50%EMS中(430 nm / min / g的OD变化3.91)。所有这些参数导致诱变剂量连续增加,导致块茎产量低。

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