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Characteristic noise features in light transmission across membrane protein undergoing photocycle

机译:在经历光循环的跨膜蛋白的光传输中的特征噪声特征

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摘要

We demonstrate a technique based on noise measurements which can be utilized to study dynamical processes in protein assembly. Direct visualization of dynamics in membrane protein system such as bacteriorhodopsin (bR) upon photostimulation are quite challenging. bR represents a model system where the stimulus-triggered structural dynamics and biological functions are directly correlated. Our method utilizes a pump-probe near field microscopy method in the transmission mode and involves analyzing the transmittance fluctuations from a finite size of molecular assembly. Probability density distributions indicating the effects of finite size and statistical correlations appear as a characteristic frequency distribution in the noise spectra of bR whose origin can be traced to photocycle kinetics. Valuable insight into the molecular processes were obtained from the noise studies of bR and its mutant D96N as a function of external parameters such as temperature, humidity or presence of an additional pump source.
机译:我们展示了一种基于噪声测量的技术,该技术可用于研究蛋白质装配中的动力学过程。在光刺激下直接可视化膜蛋白系统(如细菌视紫红质(bR))中的动力学非常具有挑战性。 bR代表一个模型系统,其中刺激触发的结构动力学和生物功能直接相关。我们的方法在传输模式下利用泵浦探针近场显微镜方法,并且涉及从有限大小的分子组装体中分析透射率波动。表示有限大小和统计相关性影响的概率密度分布作为bR噪声谱中的特征频率分布出现,其起源可以追溯到光循环动力学。通过对bR及其突变体D96N的噪声研究(取决于温度,湿度或是否存在其他泵源)的函数,可以深入了解分子过程。

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