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首页> 外文期刊>Crop Research >In vitro mass multiplication of woolly aphid (Ceratovacuna anigera Zehntner) resistant sugarcane cultivar SNK-754
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In vitro mass multiplication of woolly aphid (Ceratovacuna anigera Zehntner) resistant sugarcane cultivar SNK-754

机译：甘蔗抗性甘蔗品种SNK-754的体外大量繁殖

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摘要

Meristem culture is the routine practice in micropropagation of sugarcane using semi-solid medium. Mass multiplication of sugarcane woolly aphid resistant cultivar SNK-754 using meristem culture on liquid medium was attempted in the present study. Liquid medium was used in all the stages of micropropagation of sugarcane in combination with different growth regulators on respective stages. Sugarcane shoots from 8-month old mother plant were collected and meristem buds were taken out and sterilized before inoculation on MS medium supplemented with 0.25 mg/l BAP, 0.1 mg/l GA(3), 0.2 mg/l NAA and 20 g/l sucrose for initiation provided with a filter paper bridge as a support. Shoots emerged from meristems and subcultured for multiplication on medium consisting of MS, 0.25 mg/l BAP, 0.1 mg/l NAA and 20 g/l sucrose. The culture bottles during multiplication were kept on continuous shaking at three different speeds viz., 25, 50 and 100 RPM on orbital shaker to determine the optimum speed for multiplication of large number of shoots. The highest rate of multiplication of shoots with an average of 9.10 seedlings on each cycle of multiplication was found at 100 RPM and lowest was at 25 RPM with 3.95 seedlings per cycle of multiplication. The meristems inoculated on static position performed poorly with an average of 3.10 seedlings per cycle of multiplication. After multiplication elongated shoots were rooted on rooting medium (MS + 0.01 mg/l IBA and 20 g/l sucrose) and seedlings were hardened before discharge for field plantation. The rate of shoot multiplication was found increasing from 25 to 100 RPM revealing 100 RPM the most suitable for large scale multiplication of sugarcane using liquid culture medium on orbital shaker. The cost of production for each seedling was also found less compared to gelled medium and method was found most successful in popularizing the newly developed sugarcane in short period and viable for commercial scale multiplication of sugarcane.
机译：分生组织培养是使用半固体培养基进行甘蔗微繁殖的常规方法。在本研究中，尝试使用分生组织培养法在甘蔗抗羊毛蚜抗性品种SNK-754上大量繁殖。在甘蔗微繁殖的所有阶段，均与液态培养基结合，在各个阶段使用不同的生长调节剂。收集8个月大的母本植物的甘蔗芽，取出分生组织芽并灭菌，然后接种在补充0.25 mg / l BAP，0.1 mg / l GA（3），0.2 mg / l NAA和20 g / l的MS培养基上l蔗糖用于引发，配有滤纸桥作为支撑。从分生组织中萌出芽，并在MS，0.25 mg / l BAP，0.1 mg / l NAA和20 g / l蔗糖组成的培养基上进行继代培养以繁殖。繁殖期间，将培养瓶以三种不同的速度（即25、50和100 RPM）在轨道摇床上保持连续摇动，以确定用于繁殖大量芽的最佳速度。在每个繁殖周期中，平均每芽繁殖9.10苗的新芽的最高繁殖率是100 RPM，最低是25 RPM，每繁殖周期有3.95苗。在静止位置接种的分生组织表现较差，每个繁殖周期平均有3.10株幼苗。繁殖后，将细长的枝条生根在生根培养基（MS + 0.01 mg / l IBA和20 g / l蔗糖）上，使幼苗硬化，然后排出田间种植。发现枝条繁殖的速率从25 RPM增加到100 RPM，表明100 RPM最适合在轨道振荡器上使用液体培养基进行甘蔗的大规模繁殖。还发现与胶凝培养基相比，每株幼苗的生产成本更低，并且发现方法在短时间内推广新开发的甘蔗最成功，并且对于商业规模的甘蔗繁殖是可行的。

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