Application of fluorescent microscopic technique to interpret the pistils of diploids and tetraploids of Gossypium species

Saravanan S.; Koodalingam K.; Ramakrishnan S. Hari

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Application of fluorescent microscopic technique to interpret the pistils of diploids and tetraploids of Gossypium species

机译：荧光显微镜技术在棉属植物二倍体和四倍体雌蕊解释中的应用

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Self pollen pistils of six diploid genotypes (K 10, K 11, PA 255, DLSA 8, DL8A 16 and TKA 9410), three tetraploids (MCU 5, Sahana and MCU 12) and cross pollinated pistils of direct combination of above parents were collected at 2, 4, 6, 8, 10, 12, 24 and 36 h after pollination fixed in 6 : 3 : 1 (ethanol, chloroform, acetic acid) at 4-1 degrees C for 24 h. The softening of the pistil of diploid parents was optimum at 12N and 10N NaOH for 6 and 8 h duration, respectively, besides the tetraploids had 10N NaOH for 10 h as optimum. The optimum staining and better fluorescence were obtained when 0.25% aniline blue was dissolved in 0.1 N K3PO4 and kept for 10 h for both diploid and tetraploid.

机译：收集了六种二倍体基因型（K 10，K 11，PA 255，DLSA 8，DL8A 16和TKA 9410），三个四倍体（MCU 5，Sahana和MCU 12）的自花粉雌蕊和上述亲本的直接组合的交叉授粉雌蕊。授粉后的2、4、6、8、10、12、24和36 h在4-1摄氏度的6：3：1（乙醇，氯仿，乙酸）中固定24 h。二倍体亲代雌蕊的软化在12N和10N NaOH下分别持续6h和8h最佳，而四倍体的10N NaOH最佳持续10h。当将0.25％苯胺蓝溶于0.1 N K3PO4中并保持二倍体和四倍体均保持10 h时，可获得最佳染色和更好的荧光。

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《Crop Research》 |2009年第3期|共2页
作者
Saravanan S.; Koodalingam K.; Ramakrishnan S. Hari;
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正文语种 eng
中图分类农作物;
关键词
genotype; diploidy; tetraploidy; cross pollination;

机译：基因型;二倍体;四倍体;异花授粉;

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1. Application of fluorescent microscopic technique to interpret the pistils of diploids and tetraploids of Gossypium species [J] . Saravanan S., Koodalingam K., Ramakrishnan S. Hari Crop Research . 2009,第1a3期

机译：荧光显微镜技术在棉属植物二倍体和四倍体雌蕊解释中的应用
2. Usefulness of SSR Derived from Tetraploid Gossypium spp. for Analyses of Diploid Gossypium spp. [J] . M. Altaf-Khan, Samina N. Qureshi, Sukumar Saha, Journal of Crop Improvement . 2006,第1a2期

机译：四倍体棉属植物衍生的SSR的有用性。用于二倍体棉的分析。
3. Morphological studies on the development of transmitting tissues in diploid and tetraploid grape pistils [J] . Okamoto G, Hayashi Y, Hirano K 园芸学会杂志 . 2002,第1期

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5. Evolution of the alcohol dehydrogenase gene family in diploid and tetraploid Gossypium L. [D] . Small, Randall Lee. 1999

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6. Genome-wide analysis and comparison of the DNA-binding one zinc finger gene family in diploid and tetraploid cotton (Gossypium) [O] . YuZhe Li, Zhen Liu, KaiYue Zhang, 2020

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7. Morphological Studies on the Development of Transmitting Tissues in Diploid and Tetraploid Grape Pistils. [O] . Goro Okamoto, Yoshiko Hayashi, Ken Hirano 2002

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8. Evaluation of a Radiographic Paper System Using Ultraviolet Fluorescent Screens. Part II. Summary of Part I,Techniques and Sensitivity Data for F-2Screen,Applications. [R] . holloway, j. a. 1975

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Application of fluorescent microscopic technique to interpret the pistils of diploids and tetraploids of Gossypium species

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