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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Specific Drosophila Dscam juxtamembrane variants control dendritic elaboration and axonal arborization.
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Specific Drosophila Dscam juxtamembrane variants control dendritic elaboration and axonal arborization.

机译：果蝇Dscam近膜的特定变体控制树突状细化和轴突乔化。

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摘要

Drosophila Dscam isoforms are derived from two alternative transmembrane/juxtamembrane domains (TMs) in addition to thousands of ectodomain variants. Using a microRNA-based RNA interference technology, we selectively knocked down different subsets of Dscams containing either the exon 17.1- or exon 17.2-encoding TM. Eliminating Dscam[TM1] reduced Dscam expression but minimally affected postembryonic axonal morphogenesis. In contrast, depleting Dscam[TM2] blocked axon arborization. Further removal of Dscam[TM1] enhanced the loss-of-Dscam[TM2] axonal phenotypes. However, Dscam[TM1] primarily regulates dendritic development, as evidenced by the observations that removing Dscam[TM1] alone impeded elaboration of dendrites and that transgenic Dscam[TM1], but not Dscam[TM2], effectively rescued Dscam mutant dendritic phenotypes in mosaic organisms. These distinct Dscam functions can be attributed to the juxtamembrane regions of TMs that govern dendritic versus axonal targeting of Dscam as well. Together, we suggest that specific Drosophila Dscam juxtamembrane variants control dendritic elaboration and axonal arborization.
机译：果蝇Dscam同工型除了成千上万的胞外域变体之外，还衍生自两个替代的跨膜/近膜膜域（TM）。使用基于microRNA的RNA干扰技术，我们选择性地敲除含有外显子17.1或外显子17.2编码TM的Dscam的不同子集。消除Dscam [TM1]可减少Dscam表达，但对胚后轴突形态发生的影响最小。相反，消耗Dscam [TM2]会阻止轴突的树突化。 Dscam TM1的进一步去除增强了Dscam TM2轴突表型的丧失。但是，Dscam [TM1]主要调节树突的发育，这一观察结果表明，单独去除Dscam [TM1]会阻碍树突的形成，而转基因Dscam [TM1]（而不是Dscam [TM2]）有效地拯救了镶嵌中的Dscam突变树突表型。生物。这些不同的Dscam功能也可以归因于TM的近膜区域，这些区域也控制Dscam的树突和轴突靶向。在一起，我们建议特定的果蝇Dscam近膜变体控制树突状细化和轴突乔化。

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