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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function.
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A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function.

机译：星形胶质细胞，神经元和少突胶质细胞的转录组数据库：了解大脑发育和功能的新资源。

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Understanding the cell-cell interactions that control CNS development and function has long been limited by the lack of methods to cleanly separate neural cell types. Here we describe methods for the prospective isolation and purification of astrocytes, neurons, and oligodendrocytes from developing and mature mouse forebrain. We used FACS (fluorescent-activated cell sorting) to isolate astrocytes from transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of an S100beta promoter. Using Affymetrix GeneChip Arrays, we then created a transcriptome database of the expression levels of 20,000 genes by gene profiling these three main CNS neural cell types at various postnatal ages between postnatal day 1 (P1) and P30. This database provides a detailed global characterization and comparison of the genes expressed by acutely isolated astrocytes, neurons, and oligodendrocytes. We found that Aldh1L1 is a highly specific antigenic marker for astrocytes with a substantially broader pattern of astrocyte expression than the traditional astrocyte marker GFAP. Astrocytes were enriched in specific metabolic and lipid synthetic pathways, as well as the draper/Megf10 and Mertk/integrin alpha(v)beta5 phagocytic pathways suggesting that astrocytes are professional phagocytes. Our findings call into question the concept of a "glial" cell class as the gene profiles of astrocytes and oligodendrocytes are as dissimilar to each other as they are to neurons. This transcriptome database of acutely isolated purified astrocytes, neurons, and oligodendrocytes provides a resource to the neuroscience community by providing improved cell-type-specific markers and for better understanding of neural development, function, and disease.
机译：长期以来，由于缺乏清洁分离神经细胞类型的方法，限制控制中枢神经系统发育和功能的细胞间相互作用的认识一直受到限制。在这里，我们描述了从发展中和成熟的小鼠前脑中预期分离和纯化星形胶质细胞，神经元和少突胶质细胞的方法。我们使用FACS（荧光激活细胞分选）从在S100beta启动子控制下表达增强的绿色荧光蛋白（EGFP）的转基因小鼠分离星形胶质细胞。然后，我们使用Affymetrix GeneChip Arrays，通过对出生后第1天（P1）至P30之间不同出生年龄的这三种主要CNS神经细胞类型进行基因分析，创建了一个表达组数据库，其表达水平大于20,000个基因。该数据库提供了由急性分离的星形胶质细胞，神经元和少突胶质细胞表达的基因的详细全局特征和比较。我们发现Aldh1L1是星形胶质细胞的高度特异性抗原标记，与传统的星形胶质细胞标记GFAP相比，星形胶质细胞表达的模式实质上更广泛。星状细胞富含特定的代谢和脂质合成途径，以及draper / Megf10和Mertk / integrinα（v）beta5吞噬途径，表明星状细胞是专业的吞噬细胞。我们的发现对“神经胶质”细胞类别的概念提出了质疑，因为星形胶质细胞和少突胶质细胞的基因谱与神经元一样彼此不同。急性分离的纯化星形胶质细胞，神经元和少突胶质细胞的转录组数据库通过提供改进的细胞类型特异性标志物并更好地了解神经发育，功能和疾病，为神经科学界提供了资源。

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