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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Control of extracellular dopamine at dendrite and axon terminals.
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Control of extracellular dopamine at dendrite and axon terminals.

机译：控制树突和轴突末端的细胞外多巴胺。

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Midbrain dopamine neurons release dopamine from both axons and dendrites. The mechanism underlying release at these different sites has been proposed to differ. This study used electrochemical and electrophysiological methods to compare the time course and calcium dependence of somatodendritic dopamine release in the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc) to that of axonal dopamine release in the dorsal striatum. The amount of dopamine released in the striatum was approximately 20-fold greater than in cell body regions of the VTA or SNc. However, the calcium dependence and time to peak of the dopamine transients were similar. These results illustrate an unexpected overall similarity in the mechanisms of dopamine release in the striatum and cell body regions. To examine how diffusion regulates the time course of dopamine following release, dextran was added to the extracellular solution to slow diffusion. In the VTA, dextran slowed the rate of rise and fall of the extracellular dopamine transient as measured by fast-scan cyclic voltammetry yet did not alter the kinetics of the dopamine-dependent IPSC. Dextran failed to significantly alter the time course of the rise and fall of the dopamine transient in the striatum, suggesting a more influential role for reuptake in the striatum. The conclusion is that the time course of dopamine within the extracellular space of the VTA is dependent on both diffusion and reuptake, whereas the activation of D(2) receptors on dopamine neurons is primarily limited by reuptake.
机译：中脑多巴胺神经元从轴突和树突中释放多巴胺。已提出在这些不同位置释放的潜在机制不同。这项研究使用电化学和电生理学方法比较了腹侧被盖区（VTA）和黑质致密部（SNc）中树突状多巴胺释放的时程和钙依赖性与背侧纹状体中轴突性多巴胺的释放。在纹状体中释放的多巴胺的量比在VTA或SNc的细胞体区域中释放的多约20倍。但是，钙依赖性和达到多巴胺瞬态的峰值时间相似。这些结果说明纹状体和细胞体区域中多巴胺释放机理的出乎意料的总体相似性。为了检查释放后扩散如何调节多巴胺的时间过程，将葡聚糖添加到细胞外溶液中以减慢扩散。在VTA中，右旋糖酐减慢了通过快速扫描循环伏安法测量的细胞外多巴胺瞬变的上升和下降速度，但并未改变多巴胺依赖性IPSC的动力学。右旋糖酐未能显着改变纹状体中多巴胺瞬变上升和下降的时间过程，表明纹状体中再摄取的影响更大。结论是，多巴胺在VTA的细胞外空间内的时间过程取决于扩散和再摄取，而多巴胺神经元上D（2）受体的激活主要受再摄取限制。

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