...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Electrophysiological and morphological characteristics and synaptic connectivity of tyrosine hydroxylase-expressing neurons in adult mouse striatum.
【24h】

Electrophysiological and morphological characteristics and synaptic connectivity of tyrosine hydroxylase-expressing neurons in adult mouse striatum.

机译：成年小鼠纹状体中表达酪氨酸羟化酶的神经元的电生理和形态学特征以及突触连通性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Whole-cell recordings were obtained from tyrosine hydroxylase-expressing (TH(+)) neurons in striatal slices from bacterial artificial chromosome transgenic mice that synthesize enhanced green fluorescent protein (EGFP) selectively in neurons expressing TH transcriptional regulatory sequences. Stereological cell counting indicated that there were approximately 2700 EGFP-TH(+) neurons/striatum. Whole-cell recordings in striatal slices demonstrated that EGFP-TH(+) neurons comprise four electrophysiologically distinct neuron types whose electrophysiological properties have not been reported previously in striatum. EGFP-TH(+) neurons were identified in retrograde tracing studies as interneurons. Recordings from synaptically connected pairs of EGFP-TH(+) interneurons and spiny neurons showed that the interneurons elicited GABAergic IPSPs/IPSCs in spiny neurons powerful enough to significantly delay evoked spiking. EGFP-TH(+) interneurons responded to local or cortical stimulation with glutamatergic EPSPs. Local stimulation also elicited GABA(A) IPSPs, at least some of which arose from identified spiny neurons. Single-cell reverse transcription-PCR showed expression of VMAT1 in EGFP-TH(+) interneurons, consistent with previous suggestions that these interneurons may be dopaminergic as well as GABAergic. All four classes of interneurons were medium sized with modestly branching, varicose dendrites, and dense, highly varicose axon collateral fields. These data show for the first time that there exists in the normal rodent striatum a substantial population of TH(+)/GABAergic interneurons comprising four electrophysiologically distinct subtypes whose electrophysiological properties differ significantly from those of previously described striatal GABAergic interneurons. These interneurons are likely to play an important role in striatal function through fast GABAergic synaptic transmission in addition to, and independent of, their potential role in compensation for dopamine loss in experimental or idiopathic Parkinson's disease.
机译：全细胞记录是从细菌人工染色体转基因小鼠的纹状体切片中表达酪氨酸羟化酶（TH（+））神经元获得的，该细菌人工染色体转基因小鼠在表达TH转录调控序列的神经元中选择性地合成增强的绿色荧光蛋白（EGFP）。立体细胞计数表明，大约有2700个EGFP-TH（+）神经元/纹状体。纹状体切片中的全细胞记录表明，EGFP-TH（+）神经元包含四种电生理学上不同的神经元类型，这些类型的电生理学特性以前从未在纹状体中报道过。 EGFP-TH（+）神经元在逆行追踪研究中被鉴定为中间神经元。突触连接对的EGFP-TH（+）中间神经元和多刺神经元的记录显示，中间神经元在多刺神经元中引起GABA能IPSP / IPSC，其功能足以显着延迟诱发的尖峰。 EGFP-TH（+）interneurons对谷氨酸能EPSPs的局部或皮质刺激作出反应。局部刺激也引起了GABA（A）IPSP，其中至少一些源自确定的多刺神经元。单细胞逆转录-PCR显示VMAT1在EGFP-TH（+）中间神经元中的表达，与以前的建议一致，即这些中间神经元既可能是多巴胺能的，也可能是GABA的。所有四类中间神经元均中等大小，具有适度的分支，曲张的树突和密集的，高度曲张的轴突侧支场。这些数据首次表明，在正常啮齿动物纹状体中存在大量的TH（+）/ GABA能性中间神经元，其包含四种电生理学上不同的亚型，它们的电生理特性明显不同于先前描述的纹状体GABA能性中间神经元。这些中间神经元可能通过快速的GABA能突触传递在纹状体功能中发挥重要作用，并且独立于它们在补偿实验性或特发性帕金森氏病中多巴胺损失中的潜在作用，并且与它们无关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号