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首页> 外文期刊>The European Journal of Neuroscience >Electrophysiological and morphological characteristics of GABAergic respiratory neurons in the mouse pre-Botzinger complex.
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Electrophysiological and morphological characteristics of GABAergic respiratory neurons in the mouse pre-Botzinger complex.

机译：小鼠前Botzinger复合体中GABA能呼吸神经元的电生理和形态学特征。

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摘要

The characteristics of GABAergic neurons involved in respiratory control have not been fully understood because identification of GABAergic neurons has so far been difficult in living tissues. In the present in vitro study, we succeeded in analysing the electrophysiological as well as morphological characteristics of GABAergic neurons in the pre-Botzinger complex. We used 67-kDa isoform of glutamic acid decarboxylase-green fluorescence protein (GAD67-GFP) (Delta neo) knock-in (GAD67(GFP/+)) mice, which enabled us to identify GABAergic neurons in living tissues. We prepared medullary transverse slices that contained the pre-Botzinger complex from these neonatal mice. The fluorescence intensity of the pre-Botzinger complex region was relatively high among areas of the ventral medulla. Activities of GFP-positive neurons in the pre-Botzinger complex were recorded in a perforated whole-cell patch-clamp mode. Six of 32 GFP-positive neurons were respiratory and the remaining 26 neurons were non-respiratory; the respiratory neurons were exclusively inspiratory, receiving excitatory post-synaptic potentials during the inspiratory phase. In addition, six inspiratory and one expiratory neuron of 30 GFP-negative neurons were recorded in the pre-Botzinger complex. GFP-positive inspiratory neurons showed high membrane resistance and mild adaptation of spike frequency in response to depolarizing current pulses. GFP-positive inspiratory neurons had bipolar, triangular or crescent-shaped somata and GFP-negative inspiratory neurons had multipolar-shaped somata. The somata of GFP-positive inspiratory neurons were smaller than those of GFP-negative inspiratory neurons. We suggest that GABAergic inhibition not by expiratory neurons but by inspiratory neurons that have particular electrophysiological and morphological properties is involved in the respiratory neuronal network of the pre-Botzinger complex.
机译：到目前为止，尚未完全了解参与呼吸控制的GABA能神经元的特征，因为到目前为止，在活体组织中很难鉴定GABA能神经元。在目前的体外研究中，我们成功地分析了前Botzinger复合体中GABA能神经元的电生理和形态特征。我们使用了谷氨酸脱羧酶绿色荧光蛋白（GAD67-GFP）（Delta neo）敲入（GAD67（GFP / +））小鼠的67 kDa亚型，这使我们能够鉴定活组织中的GABA能神经元。我们准备了包含这些新生小鼠的博兹林前复合体的髓质横切片。腹侧延髓区域之间的前柏辛格复合体区域的荧光强度相对较高。以穿孔的全细胞膜片钳模式记录了前Botzinger复合物中GFP阳性神经元的活性。 32个GFP阳性神经元中有6个是呼吸性的，其余26个神经元是非呼吸性的。呼吸神经元完全是吸气的，在吸气阶段具有兴奋性的突触后电位。此外，在Botzinger前复合体中记录了30个GFP阴性神经元中的6个吸气和1个呼气神经元。 GFP阳性吸气神经元对去极化电流脉冲表现出较高的膜电阻和对尖峰频率的适度适应。 GFP阳性吸气神经元具有双极，三角形或新月形的躯体，而GFP阴性吸气神经元具有多极形的躯体。 GFP阳性吸气神经元的体细胞比GFP阴性吸气神经元的体细胞小。我们建议，GABA能的抑制不是由呼气神经元而是由具有特定电生理和形态学特性的吸气神经元引起的，其参与了前Botzinger复合体的呼吸神经元网络。

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