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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Stereological estimate of the total number of neurons in spinal segment D9 of the red-eared turtle.
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Stereological estimate of the total number of neurons in spinal segment D9 of the red-eared turtle.

机译：红耳龟脊髓节段D9中神经元总数的体视学估计。

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摘要

The red-eared turtle is an important animal model for investigating the neural activity in the spinal circuit that generates motor behavior. However, basic anatomical features, including the number of neurons in the spinal segments involved, are unknown. In the present study, we estimate the total number of neurons in segment D9 of the spinal cord in the red-eared turtle (Trachemys scripta elegans) using stereological cell counting methods. In transverse spinal cord sections stained with modified Giemsa, motoneurons (MNs), interneurons (INs), and non-neuronal cells were distinguished according to location and morphology. Each cell type was then counted separately using an optical disector with the cell nucleus as counting item. The number of cells in segment D9 was as follows (mean +/- SE): MNs, 2049 +/- 74; INs, 16,135 +/- 316; non-neuronal cells, 47,504 +/- 478 (n = 6). These results provide the first estimate of the total number of neurons in a spinal segment in a terrestrial vertebrate based on unbiased stereological methods and an upper bound on the number of neurons involved in segmental sensorimotor activity. These findings also form a crucial quantitative foundation for integrating electrophysiological data into mathematical circuit models.
机译：红耳龟是一种重要的动物模型，用于研究产生运动行为的脊髓回路中的神经活动。但是，基本的解剖特征（包括所涉及的脊柱节段中的神经元数量）是未知的。在本研究中，我们使用立体细胞计数方法估算了红耳龟（Trachemys scripta elegans）脊髓D9段的神经元总数。在经修饰的吉姆萨染色的横断脊髓切片中，根据位置和形态区分运动神经元（MN），中间神经元（IN）和非神经元细胞。然后使用光学解剖仪以细胞核作为计数项目分别计数每种细胞类型。段D9中的细胞数目如下（平均+/- SE）：MN，2049 +/- 74; MNs，2049 +/- 74; MNs，2049 +/- 74。 IN，16,135 +/- 316;非神经元细胞47,504 +/- 478（n = 6）。这些结果基于无偏见的立体学方法提供了陆生脊椎动物脊柱节段中神经元总数的第一个估计值，并且提供了涉及节段感觉运动活动的神经元数的上限。这些发现也为将电生理数据整合到数学电路模型中奠定了至关重要的定量基础。

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